Detection method of zeaxanthin mono-cis and di-cis isomers
A technology of zeaxanthin and detection method, which is applied in the field of liquid chromatography analysis, can solve the problems of separation and detection of zeaxanthin bis-cis isomers, etc., and achieve the effects of high sensitivity, good reproducibility, simple and economical method
Active Publication Date: 2015-11-25
JIANGSU ACAD OF AGRI SCI
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Problems solved by technology
It has been reported to separate the cis-isomers of lutein and α, β-carotene, but there is no related report on the separation and detection of the di-cis-isomers of zeaxanthin
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[0034] The following examples can better understand the present invention, but do not limit the present invention in any way.
[0035] 1. Experimental instruments and reagents
[0036] (1) Experimental equipment
[0037] Agilent6530 quadrupole-time-of-flight tandem mass spectrometer (6530Q-TOF, APCI source), Agilent1200HPLC chromatograph (Agilent, USA), YMC-C30 column (YMC, Japan), D10 nitrogen purging instrument (Hangzhou Aosheng Instrument Co., Ltd.)
[0038] (2) Experimental reagents
[0039] All-trans zeaxanthin standard substance (purity 97%), American Sigma company
[0040] Chromatographic grade methanol, methyl tert-butyl ether (American Tiandi Company)
[0041] 2. Experimental method
[0042] (1) Preparation of zeaxanthin single-cis and double-cis isomers
[0043] Dissolve 1.0 mg of all-trans zeaxanthin standard substance in methanol and dilute it to a 25 mL brown volumetric flask, and place it in an ultra-low temperature refrigerator for use. Take 6 parts of 2 mL ...
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Abstract
The invention relates to a method for detecting mono-cis / bicis-isomer of zeaxanthin, and belongs to the technical field of analysis. The method comprises the following steps: performing isomerization reaction on all-trans zeaxanthin photo-iodine to prepare the single-cis / bicis-isomer; remarkably separating the single-cis / bicis-isomer of zeaxanthin by using a YMCCarotenoid C30 chromatographic column, wherein the ratio of a flow phase of methanol to methyl tertiary butyl ether to water is 80:15:5, the ratio of methanol to methyl tertiary butyl ether is 1:10, the flowing speed is 1.0mL / min, the temperature of the chromatographic column is 25 DEG C, the ultraviolet detection wavelength is 450nm, the feeding amount is 20 micro-Ls, an ion source APCi+ is adopted, the flowing speed of a component flowing out the chromatographic column and entering a mass spectrometer is 10 micro-L / min, the scanning range is 80-1000m / z, the capillary tube voltage is 2500V, 5 liters of drying gas is adopted, 20psi of atomizing gas is adopted, the atomization temperature is 300 DEG C, the steam temperature is 400 DEG C, and the corona current is 4 micro-A; and performing qualitative analysis according to the retention time, a chromatogram and a spectrogram of a sample, and performing quantitative analysis according to a peak area. The method has the advantages of high sensitivity, good reproducibility and high recycling rate, and is applicable to qualitative and quantitative analysis on the all-trans, mono-cis and bicis-isomer of the zeaxanthin.
Description
1. Technical field [0001] The invention relates to a detection method of zeaxanthin single-cis and double-cis isomers, belonging to the technical field of liquid chromatography analysis. 2. Background technology [0002] Zeaxanthin, also known as zeaxanthin, is an oil-soluble natural pigment widely found in green leafy vegetables, flowers, fruits, wolfberry and yellow corn. There are 11 conjugated double bonds in the chemical structure of zeaxanthin, and there are hydroxyl groups on the tail group. This specific structure makes it have a strong ability to quench singlet oxygen and scavenge oxygen free radicals, which can effectively prevent and Reduce oxidative damage to the human retina and protect the eyes. In addition, zeaxanthin has significant physiological effects in reducing the incidence of cardiovascular diseases, enhancing immune function and reducing the occurrence and development of cancer. [0003] Zeaxanthin in nature is mainly in the all-trans structure. Due...
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IPC IPC(8): G01N30/88
Inventor 张钟元李大婧肖亚冬刘春泉
Owner JIANGSU ACAD OF AGRI SCI