Method for purifying RNA on preparative scale by means of HPLC

An alkylation and mobile phase technology, applied in DNA preparation, recombinant DNA technology, organic chemistry, etc., can solve complex and expensive gradient procedures and other problems, and achieve the effects of simple recovery, sensitive detection, and easy automatic operation
CN101563457BActive Publication Date: 2014-03-12CUREVAC REAL ESTATE GMBH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CUREVAC REAL ESTATE GMBH
Publication Date
2014-03-12

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Abstract

The application describes a method for the preparative purification of RNA, which method is distinguished in that the RNA is purified by means of HPLC using a porous reversed phase as the stationary phase. The use of the porous reversed phase in this HPLC method is also described.
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Description

technical field

[0001] The present invention relates to a method for the purification of RNA by HPLC on a preparative scale (preparative scale) and the use of a porous reversed phase as a stationary phase in this method. Background technique

[0002] HPLC (short for "High Performance (High Pressure) Liquid Chromatography") is an established method for separating mixtures of substances that is widely used in biochemistry, analytical chemistry, and clinical chemistry.

[0003] In the simplest case, an HPLC setup consists of a pump with an eluent reservoir (filled with mobile phase), a sample injection system, a separation column filled with a stationary phase, and a detector. Furthermore, a fraction collector can also be provided, with which individual fractions can be collected in each case after separation and can thus be used in further applications.

[0004] RNA analysis using ion-pair reverse-phase HPLC is known from A. Azarani and K. H. Hecker (Nucleic Acids Research, v...

Claims

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