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Detection method for single-cis and double-cis lutein isomers

A detection method, double-cis technology, applied in the field of analysis, can solve the problems of detection of lutein double-cis isomers that have not yet been seen, and achieve the effects of improved tailing phenomenon, good peak shape, high accuracy and sensitivity

Active Publication Date: 2014-10-08
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report on the detection of lutein dicis-isomers

Method used

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  • Detection method for single-cis and double-cis lutein isomers
  • Detection method for single-cis and double-cis lutein isomers
  • Detection method for single-cis and double-cis lutein isomers

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Embodiment Construction

[0032] The following examples can better understand the present invention, but do not limit the present invention in any way.

[0033] 1. Experimental instruments and reagents

[0034] 1) Experimental equipment

[0035] Agilent 6530 quadrupole-time-of-flight tandem mass spectrometer (6530Q-TOF, APCI source), Agilent 1200HPLC chromatograph (Agilent, USA), YMC-C30 column (YMC, Japan)

[0036] D10 nitrogen purging instrument (Hangzhou Aosheng Instrument Co., Ltd.).

[0037] 2) Experimental reagents

[0038] All-trans lutein standard substance (purity 97%), U.S. Sigma company;

[0039] Chromatographic grade methanol, acetonitrile, American Tiandi Company;

[0040] Chromatographic grade dichloromethane, American ACS Enke Chemicals.

[0041] 2. Experimental method

[0042] 1) Preparation of samples of lutein mono-cis and di-cis isomers

[0043] Draw 4.6mL of soybean oil into a stoppered graduated test tube, and heat in a constant temperature oil bath at 170°C in the dark. We...

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Abstract

The invention discloses a detection method for single-cis and double-cis lutein isomers. The method adopts a diode array detector and a YMC carotenoid C30 chromatographic column, and takes methyl alcohol, acetonitrile and dichlormethane as mobile phases, wherein the ratio of methyl alcohol to acetonitrile to dichlormethane is 50 to 30 to 20; according to the method, the time is 13 minutes, the flow velocity is 1.0mL / minute, the column temperature is 25 DEG C, the sample injection amount is 20 microliters, the scanning wavelength is 300-500nm, and the detection wavelength is 450nm; after the method is adopted, the single-cis and double-cis lutein isomers can be well separated; furthermore, an atmospheric pressure chemical ionization (APCI) positive ion mass spectrum is utilized, the flow velocity of chromatographic column outflow components entering a mass spectrometer is 10 microliters per minute, the scanning range m / z=80-900, the vaporization temperature is 350 DEG C, the flow velocity of dry gas is 5L / minute, the corona current is 4 microamperes, the crushing voltage is 150 V, and the capillary voltage is 2,500V. According to the mass spectrum and optical spectrum information, the single-cis and double-cis lutein isomers are respectively determined to be 13, 15-double-cis form, 9, 15-double-cis form, 15-cis form, 13-cis form, 13'-cis form, 9, 9'-double-cis form, 9-cis form and 9'-cis form lutein. The method has the advantages of being rapid, effective, good in reproducibility, high in recycling rate and the like, and can be used for carrying out qualitative and quantitative analysis on the single-cis and double-cis lutein isomers.

Description

1. Technical field [0001] The invention relates to a detection method for lutein mono-cis and bi-cis isomers, belonging to the technical field of analysis. 2. Background technology [0002] Lutein, also known as "plant lutein", is a V-free A Active oxygen-containing carotenoids widely exist in vegetables, flowers, fruits and some algae. More than ten years of scientific research has shown that lutein is the main component of macular pigment in the human eye. Lutein can not only filter the blue light that has the strongest effect on macular damage, but also quench and capture the active oxygen free radicals induced by high-energy blue light, thereby protecting vision and anti-oxidation. Therefore, regular supplementation of lutein can reduce the risk of age-related macular degeneration (Age-related Macular Degeneration, referred to as AMD). In addition, lutein also has physiological functions such as preventing cataract and human aging, anti-cancer, anti-mutagenesis, and d...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 李大婧肖亚冬张钟元刘春泉
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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