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Detection method for evaluating irritation of traditional Chinese medicine

A detection method and stimulation technology, applied in the detection field, can solve problems such as inability to effectively detect the stimulation of traditional Chinese medicine

Inactive Publication Date: 2014-10-22
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: the technical problem to be solved by this invention is to overcome the deficiency that the prior art cannot effectively detect the irritation of traditional Chinese medicines, and to provide a universal, intuitive, convenient and fast evaluation method for the irritation of traditional Chinese medicines

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Irritation detection of toad venom crude drug

[0020] 1. Method

[0021] 1. Extraction and preparation of toad venom crude drug

[0022] Weigh 0.3492g of the crude drug powder of toadstool and place it in a glass test tube, add 8ml of 0.5% DMSO ultrapure water for ultrasonic extraction twice, 20min each time, filter, and combine the filtrates. The filtrate was freeze-dried to a powder state, weighing 0.1211g, that is, the yield was 34.68%. Take 0.0419g of the freeze-dried powder and dissolve it in 0.419ml of 0.5% DMSO water to obtain a mother liquor with a concentration of 100mg / ml, filter it, and prepare solutions with a concentration of 10mg / ml, 20mg / ml, and 50mg / ml respectively That's it.

[0023] 2. Rat foot irritation test

[0024] 40 male SD rats were selected and randomly divided into 4 groups, ie normal group (normal saline), administration group (10mg / ml, 20mg / ml, 50mg / ml), 10 in each group.

[0025] A mark was made at the ankle joint of the rig...

Embodiment 2

[0035] The irritation detection of embodiment 2 mylabris

[0036] 1. Method

[0037] 1. The crude medicine of Mylabris

[0038] The crude drug concentration of Mylabris is 0.03g / ml, provided by Beijing Zhengbang Pharmaceutical Co., Ltd.

[0039] 2. Rat foot irritation test

[0040] 20 male SD rats were randomly divided into 2 groups, namely the normal group (normal saline) and the administration group (0.03g / ml), 10 in each group.

[0041] A mark was made at the ankle joint of the right hindlimb of the rats in each group, and the volume of the right hindpaw was measured by the drainage method. Inject the above two groups of different drugs into the right hind foot pad of the rats, each rat injects 0.1ml, observe the rat's foot-lifting response, record the number of times the rat's foot is lifted within 30 minutes, and measure the rat's 30-minute drug injection. The volume of the right hind paw was calculated, and the volume difference before and after the experiment of the...

Embodiment 3

[0051] Example 3 Irritation Detection of Cinobufacin Injection

[0052] 1. Method

[0053] 1. Collection of irritating parts of traditional Chinese medicine injection

[0054] Take 20ml of cinobufacin injection, put it into a 3KD ultrafiltration tube, centrifuge at 3500 rpm for 40min, and undergo repeated ultrafiltration to obtain 10 times concentrated and enriched macromolecules and the ultrafiltration lower layer, and store at 4 degrees.

[0055] Take 5ml of cinobufacin injection, dilute it by 1 time, and store it at 4°C.

[0056] 2. Rat foot irritation test

[0057] 50 male SD rats were randomly divided into 5 groups, i.e. normal group (normal saline), administration group (cinobufacin injection stock solution, cinobufacin injection 0.5 times concentrated solution, 10 times concentrated solution, 10 times concentrated solution lower layer), 10 rats in each group.

[0058] A mark was made at the ankle joint of the right hindlimb of the rats in each group, and the volume ...

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Abstract

The invention provides a detection method for evaluating irritation of a traditional Chinese medicine. According to the detection method for evaluating the irritation of the traditional Chinese medicine, traditional Chinese medicine stock solution or extracting solution is subcutaneously injected to toes of an experimental animal, foot lifting frequency of an animal is used for reflecting pain caused by irritation of the traditional Chinese medicine, degree of swelling is used for reflecting inflammation caused by the irritation of the traditional Chinese medicine, and the irritation of the traditional Chinese medicine is comprehensively evaluated according to the pain and the inflammation. Through verification to two crude drugs and two injections, namely venenum bufonis, Chinese blister beetle, cinobufagin injection and DeLiSheng injection, results show that the detection method can effectively evaluate irritating intensity of the traditional Chinese medicine. The detection method for evaluating the irritation of the traditional Chinese medicine sensitive, visible, simple, convenient and rapid and can be widely applied to evaluation and verification of the irritations of various traditional Chinese medicines.

Description

technical field [0001] The invention relates to a detection method, specifically designing a detection method widely applicable to the evaluation of the irritation of traditional Chinese medicines. Background technique [0002] As an effective means of preventing and treating diseases, traditional Chinese medicine conforms to the new trend of medical development in the 21st century, and shows strong vitality and broad development prospects. The clinical use of traditional Chinese medicine in the current Chinese and Western hospitals is also increasing significantly, especially the ranking of traditional Chinese medicine in the clinical innovative drugs of Western hospitals has been greatly improved. The sales of traditional Chinese medicines have been increasing in recent years. For example, Sanqi in Wenshan City has annual sales of nearly 5 billion. [0003] However, traditional Chinese medicine often has adverse reactions when it is used clinically, which has potential sa...

Claims

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Application Information

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IPC IPC(8): A61K49/00
Inventor 马宏跃段金廒龚艳曹琴钱大玮王子月
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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