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Polynucleotide sequence for regulating low-temperature sweetening of potatoes and application thereof

A polynucleotide and low-temperature saccharification technology, which is applied in the field of plant genetic engineering, can solve the problem of no relevant literature reports, etc., and achieve the effect of improving the ability to resist low-temperature saccharification and reducing the content

Inactive Publication Date: 2014-10-29
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Currently, about StBAM1-StBAM9 There is no relevant literature report on the role of potato tuber in low temperature saccharification metabolism

Method used

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  • Polynucleotide sequence for regulating low-temperature sweetening of potatoes and application thereof
  • Polynucleotide sequence for regulating low-temperature sweetening of potatoes and application thereof
  • Polynucleotide sequence for regulating low-temperature sweetening of potatoes and application thereof

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Embodiment Construction

[0023] The present invention will be described in more detail below in conjunction with specific embodiments. From the following description and these Examples, one skilled in the art can ascertain the essential characteristics of the present invention. And without departing from the spirit and scope of the present invention, various changes and modifications can be made to the present invention to adapt it to various usages and conditions. The present invention intervenes simultaneously in potato StBAM1 with StBAM9 These two genes significantly improved the ability of potato tubers to resist low-temperature saccharification, and the effect was significantly better than that of individual intervention StAmy23 , StBAM1, StBAM9 transgenic lines. Simultaneous interference StBAM1 , StBAM9 The application of these two genes in the aspects of resistance to low-temperature saccharification and frying processing quality all belong to the protection scope of the present inven...

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Abstract

The invention discloses a polynucleotide sequence regulating low-temperature sweetening of potatoes and an application of the polynucleotide sequence. The polynucleotide sequence is shown in SEQ ID NO:1 in a sequence table. According to the invention, after the polynucleotide sequence is obtained through two genes StBAM1 and StBAM9, the two genes StBAM1 and StBAM9 are simultaneously intervened in potato, so that the content of reducing sugar can be very remarkably reduced and the low-temperature sweetening resistance of potato can be improved. The function of the polynucleotide sequence is far higher than the respective functions of StAmy23, StBAM1 and StBAM9 in low-temperature sweetening.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and specifically relates to a polynucleotide sequence for regulating low-temperature saccharification of potatoes, and its application in improving the ability of potato tubers to resist low-temperature saccharification and breeding excellent processing varieties. Background technique [0002] potato( Solanum tuberosum L.) is the fourth largest food crop in the world after rice, wheat and corn. It is also a vegetable, feed and industrial raw material crop, and is widely distributed and planted in many countries and regions in the world. With my country's economic development and industrial structure adjustment, the proportion of potatoes used as food is gradually decreasing, while the proportion of potatoes as fast food and processed products has increased significantly. [0003] Potato tubers for processing, in addition to the requirements of ordinary potatoes for rich nutrit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/84C12N15/82C12N1/21C12N15/56C12R1/19C12R1/01
Inventor 宋波涛侯娟谢从华柳俊
Owner HUAZHONG AGRI UNIV
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