Gold standard test card batch reader and test method

Abstract

A disclosed batch reader for a gold-label detection card comprises a microcomputer assembly, a CCD image scanning module performing image acquisition on the detection card, a detection card template disposed on the CCD image scanning module, an image analysis module which is connected with the microcomputer assembly and is used to analyze acquired image information; the detection card is disposed at a positioning hole of the detection card template; and the microcomputer assembly controls the CCD image scanning module and the image analysis module. A test method comprises the steps: firstly performing geometrical positioning on positions of T line and C line; determining a pre-acquisition scope according to the geometrical positioning; determining the effective pixel scope of T line and C line through comparison and calculation; and then analyzing pixel in the effective scope, and calculating the signal values of T line and C line. The batch reader and the method are applicable to reading of a large batch of colloidal gold detection cards, and also have relatively high colloidal-gold detection accuracy.

Description

technical field [0001] The invention relates to a detection device, in particular to a colloidal gold rapid detection card reading device and a testing method for rapid detection of food safety. Background technique [0002] The gold label detection card is to coat and fix the specific detection reagent antigen or antibody on the membrane in the form of a strip, and the colloidal gold-labeled reagent antibody or monoclonal antibody is adsorbed on the binding pad. When the sample to be tested is added to the test card After the sample pad at one end of the paper strip is placed on the pad, it moves forward through capillary action, dissolves the colloidal gold-labeled reagent on the pad and reacts with each other, and then moves to the area of ​​the fixed antigen or antibody, the conjugate of the test substance and the gold-labeled reagent is released again. It is trapped by its characteristic combination and gathers on the detection zone. It can be observed with the naked ey...

AI Technical Summary

Problems solved by technology

[0004] Although the existing gold standard test card batch reader solves the interpretation error caused by the naked eye judgment test results in the past, it can only place one test card at a time, and it takes a certain reaction time after the sample to be tested is added to the test card. For example, clenbuterol needs to be dripped into the sample to react for 3-5 minutes before the detection can be started. Therefore, the detection efficiency of a single card reader is low and cannot meet the detection needs of large batches of samples

[0005] Due to the different production processes of different manufacturers, the appearance size, effective reaction area, and the position and color of the reaction marking line of the gold standard detection card of different manufacturers are quite different, and the image acquisition sensors of the existing readers are generally dot matrix. It is difficult for the CCD image collector to accurately read all the effective pixels of the marking lines of different specifications of the detection card

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