Ultra-low temperature preservation and grafting resurrection of dormant buds of mulberry
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A technology of ultra-low temperature preservation and dormant buds, which is applied in the fields of plant preservation, botanical equipment and methods, and applications, and achieves the effects of good cryopreservation effect, easy operation and simple procedure.
Active Publication Date: 2016-06-29
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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So far, no universal cryopreservation method has been established in the world, which is also a major bottleneck in the practical application of cryopreservation technology
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Embodiment 1
[0027] Example 1 The relationship between the drying treatment time, water content and cryopreservation survival rate of dormant buds of different varieties
[0028] Experimental materials: The mulberry varieties used in the experiment are "Husang 32", "Yu 71-1" and "Zhongsang 9703".
[0029] The specific steps of the experimental method are as follows:
[0030] Acquisition of dormant branches: On December 27, 2013, annual new branches with normal dormant buds were cut from healthy mulberry trees in the "National Mulberry Germplasm Garden" of the Institute of Sericulture, Chinese Academy of Agricultural Sciences. During the dormancy period, the dormancy has not been broken, that is, the local temperature has not exceeded 12°C after entering continuous dormancy. For photos of dormant branches of mulberry trees retrieved from the field see figure 1 .
[0031] Cut into dormant bud segments: select the middle section of dormant branches and cut into 4-5cm long segments with 2 b...
Embodiment 2
[0042] Embodiment 2 The relationship between the physiological state of dormant bud segment and the survival rate after cryopreservation
[0043] Only the sampling time was changed (i.e. sampling on January 16, 2014 and February 17, 2014), and the rest of the steps were the same as in Example 1 to study the relationship between the physiological state of the dormant bud segment and the survival rate after cryopreservation. The results of cryopreservation are shown in Table 2.
[0044] Table 2 Relationship between physiological state and survival rate after cryopreservation
[0045]
[0046]
[0047]It can be seen from Table 2 that among the 35 treatments taken twice on January 16, 2014 and February 17, 2014, only 6 treatments survived, and the survival rate was relatively low. Because continuous high temperature occurred between the first sampling on December 27, 2013, the second sampling on January 16, 2014, and the third sampling on February 17, 2014, the dormant stat...
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Abstract
The invention relates to the field of protection of plant germplasm resources, and in particular provides a method for ultra-low temperature preservation and grafting resurrection of dormant buds of mulberry trees, including 1) obtaining dormant branches; 2) cutting into dormant buds; 3) drying; 4) sealing; 5) Gradient cooling; 6) Liquid nitrogen preservation; (7) Thawing; 8) Rehumidification; 9) Grafting resurrection. The method of the invention is a method for ultra-low temperature preservation and grafting resurrection of dormant buds of mulberry trees by using a drying-gradient cooling and freezing method. The dormant buds of mulberry trees are dried to a water content of 20%-40%, and a higher survival rate is obtained through steps such as slow and step-by-step cooling treatment, freezing storage, thawing, rehumidification, and grafting and resurrection. By adopting the method provided by the invention, the average survival rate of dormant buds of mulberry trees after cryopreservation can reach up to 95%. The cryopreservation method for mulberry dormant bud segments provided by the invention has simple procedure, easy operation and good freezing effect, and is an effective way for long-term preservation of mulberry germplasm resources in vitro.
Description
technical field [0001] The invention relates to the field of protection of plant germplasm resources, in particular to a method for ultra-low temperature preservation and grafting resurrection of dormant buds of mulberry trees. Background technique [0002] Mulberry is a dicotyledon, Moraceae, Morus (Morusalba L.) perennial woody plant (deciduous tree or shrub). It has high economic value, medicinal value and garden ornamental value. Mulberry wood is hard and can be used to make furniture, musical instruments, carvings, etc. It can also be used to make bows (mulberry arcs), papermaking, and agricultural production tools, such as mulberry branches and shafts. Dead branches can be used as dry firewood; bark can be used as medicinal materials or papermaking; mulberry leaves are the main feed for silkworms, as well as medicinal and soil pesticides. Mulberry fruit can not only satisfy hunger, but also make wine, which is called mulberry wine. The mulberry tree has a wide canop...
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