Molecular marker linked with turnip clubroot resistant gene and method for obtaining molecular marker

Abstract

The invention discloses a molecular marker linked with a turnip clubroot resistant gene and a method for obtaining the molecular marker. The method comprises the following steps: hybridizing a clubroot resistant turnip parent material P1('077-03') and an infected parent material P2('077-04') to obtain a population F1 and constructing corresponding populations BC1, F2 and F3; clearly determining that the pathogeny of the disease is plasmodiophora brassicae woron.; according to a disease-resistant genetic analysis result, indicating that the resistance of the disease is controlled by a dominant single gene; and performing clubroot resistant marker screening on the turnip population F2 by use of the SSR molecular marker technology and the improved BSA method, thereby obtaining the molecular marker BrSSR133 linked with the turnip clubroot resistant gene; the genetic distance between the SSR molecular marker and the resistance gene is 5.02cm. The research result lays a foundation for separating out the new clubroot resistant gene; the molecular marker and the method for obtaining the molecular marker are significant for both breeding practices and disease-resistant theoretical research of compestris crops.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular marker linked with a turnip clubroot disease resistance gene and an obtaining method thereof. Background technique

[0002] Cruciferous crop clubroot, also known as "root cancer", is a worldwide soil-borne disease caused by Plasmodiophora brassicae Woron. At present, it is seriously endangered in the world, and the cruciferous crops in most areas of our country are also facing the serious threat of clubroot. When cruciferous crops are infected by Plasmodium fungus, the yield will decrease significantly, or even fail. At present, chemical agents are generally used for prevention and control in production, but the effect of using agents after symptoms appear is not obvious, which brings great difficulties to the prevention and control. Studies have shown that the lifespan of dormant spores of Phizobia in soil is as long as 8-10 years, which seriously affects t...

Examples

Embodiment Construction

[0019] The method for obtaining the anti-clubbing molecular marker BrSSR133 of turnip mainly comprises the following steps:

[0020] 1. Using the clubroot resistant turnip parent material P 1 ('077-03') and susceptible parent material P 2 ('077-04'), the disease-resistant parent '077-03' and the susceptible parent '077-04' were crossed to obtain F 1 population, and construct the corresponding BC through testcross and selfcross pathways 1 , F 2 and F 3 group.

[0021] 2. According to the disease symptoms of the naturally occurring materials in the field and the microscopic examination of the dormant spores of the pathogen, it is clear that the pathogen of the disease is Plasmodium brassicae.

[0022] The specific method is: observe the disease symptoms of natural diseased materials in the field, isolate the dormant spores of the pathogen from the diseased roots and soil, and observe the morphological characteristics of the dormant spores under a 600-fold optical microscope...

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular marker linked with a turnip clubroot disease resistance gene and an obtaining method thereof. Background technique

[0002] Cruciferous crop clubroot, also known as "root cancer", is a worldwide soil-borne disease caused by Plasmodiophora brassicae Woron. At present, it is seriously endangered in the world, and the cruciferous crops in most areas of our country are also facing the serious threat of clubroot. When cruciferous crops are infected by Plasmodium fungus, the yield will decrease significantly, or even fail. At present, chemical agents are generally used for prevention and control in production, but the effect of using agents after symptoms appear is not obvious, which brings great difficulties to the prevention and control. Studies have shown that the lifespan of dormant spores of Phizobia in soil is as long as 8-10 years, which seriously affects t...

Examples

Embodiment Construction

[0019] The method for obtaining the anti-clubbing molecular marker BrSSR133 of turnip mainly comprises the following steps:

[0020] 1. Using the clubroot resistant turnip parent material P 1 ('077-03') and susceptible parent material P 2 ('077-04'), the disease-resistant parent '077-03' and the susceptible parent '077-04' were crossed to obtain F 1 population, and construct the corresponding BC through testcross and selfcross pathways 1 , F 2 and F 3 group.

[0021] 2. According to the disease symptoms of the naturally occurring materials in the field and the microscopic examination of the dormant spores of the pathogen, it is clear that the pathogen of the disease is Plasmodium brassicae.

[0022] The specific method is: observe the disease symptoms of natural diseased materials in the field, isolate the dormant spores of the pathogen from the diseased roots and soil, and observe the morphological characteristics of the dormant spores under a 600-fold optical microscope...

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