Molecular marker linked with turnip clubroot resistant gene and method for obtaining molecular marker

Abstract

The invention discloses a molecular marker linked with a turnip clubroot resistant gene and a method for obtaining the molecular marker. The method comprises the following steps: hybridizing a clubroot resistant turnip parent material P1('077-03') and an infected parent material P2('077-04') to obtain a population F1 and constructing corresponding populations BC1, F2 and F3; clearly determining that the pathogeny of the disease is plasmodiophora brassicae woron.; according to a disease-resistant genetic analysis result, indicating that the resistance of the disease is controlled by a dominant single gene; and performing clubroot resistant marker screening on the turnip population F2 by use of the SSR molecular marker technology and the improved BSA method, thereby obtaining the molecular marker BrSSR133 linked with the turnip clubroot resistant gene; the genetic distance between the SSR molecular marker and the resistance gene is 5.02cm. The research result lays a foundation for separating out the new clubroot resistant gene; the molecular marker and the method for obtaining the molecular marker are significant for both breeding practices and disease-resistant theoretical research of compestris crops.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular marker linked with a turnip clubroot disease resistance gene and an obtaining method thereof. Background technique [0002] Cruciferous crop clubroot, also known as "root cancer", is a worldwide soil-borne disease caused by Plasmodiophora brassicae Woron. At present, it is seriously endangered in the world, and the cruciferous crops in most areas of our country are also facing the serious threat of clubroot. When cruciferous crops are infected by Plasmodium fungus, the yield will decrease significantly, or even fail. At present, chemical agents are generally used for prevention and control in production, but the effect of using agents after symptoms appear is not obvious, which brings great difficulties to the prevention and control. Studies have shown that the lifespan of dormant spores of Phizobia in soil is as long as 8-10 years, which seriously affects t...

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Problems solved by technology

[0003] In order to solve the above-mentioned major problems affecting the sustainable development of cruciferous crops in my country and discover new antigens, breeders in the past mostly used morphological markers and biochemical markers to assist breeding, and some excellent clubroot-resistant selfings have been obtained after screening. However, in the process of disease-resistant transgenics, there are disadvantages such as long selection time and cumbersome operation procedures when using traditional methods to screen resistant plants; on the other hand, resistance performance is easily affected by environmental conditions, and it is difficult to adapt to breeding practices. need

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