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Molecular marker linked with paddy nitrogen nutrition regulation and control gene and application thereof

A technology of molecular markers and gene regulation, applied in the field of agricultural biology, can solve the problems of nutrient loss, waste of nutrient resources, serious environmental pollution, etc., achieve high nitrogen utilization and regulation ability, and overcome the effect of long time period

Inactive Publication Date: 2010-08-18
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the problem of environmental pollution caused by high nitrogen is becoming more and more serious
According to statistics, the average utilization rate of nitrogen fertilizer in my country is only 35%. Nutrient loss not only seriously wastes limited nutrient resources, but also brings worrying environmental problems due to its discharge to water bodies and the atmosphere.

Method used

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  • Molecular marker linked with paddy nitrogen nutrition regulation and control gene and application thereof
  • Molecular marker linked with paddy nitrogen nutrition regulation and control gene and application thereof
  • Molecular marker linked with paddy nitrogen nutrition regulation and control gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Using SSR Molecular Markers to Obtain the Molecular Marker RM5897 Linked to Nitrogen Regulatory Genes in Rice

[0039] Plant material: The nitrogen-efficient gene donor parent was an indica rice variety Zhaiyeqing 8 (ZYQ8) from the China Rice Research Institute, which was crossed with a common japonica rice variety Jingxi 17 (JX17). 1 The doubling haploid (DH) population was obtained by cultured anthers, and the nitrogen-efficient strain DH78 was obtained from it. Through crossing, backcrossing and selfing of DH78 and JX17, grouping and gene mapping were carried out, and 87 strains were identified from the offspring of this population. Individuals with similar DH78 phenotypes were used for linkage analysis.

[0040] (1) DNA extraction

[0041] First prepare the DNA extraction buffer, add 1 volume of DNA extraction solution (0.35M sorbitol; 0.1M Tris, pH8.2; 0.005M EDTA) and 1 volume of nuclear lysate (0.2M Tris, pH7.5; 0.05M EDTA; 2M NaCl; 0.055M CTAB) and 0.4 volumes...

Embodiment 2

[0056] Using STS Molecular Markers to Obtain Molecular Markers Linked to Regulatory Genes of Nitrogen Nutrition in Rice

[0057] AP5756

[0058] Plant material is with embodiment 1, and concrete way is as follows:

[0059] (1) DNA extraction

[0060] First prepare the DNA extraction buffer, add 1 volume of DNA extraction solution (0.35M sorbitol; 0.1M Tris, pH8.2; 0.005M EDTA) and 1 volume of nuclear lysate (0.2M Tris, pH7.5; 0.05M EDTA; 2M NaCl; 0.055M CTAB) and 0.4 volumes of 5% sarkosyl, with sodium bisulfite added to a final concentration of 0.02M. Weigh 0.1 g of rice leaves and grind them into powder with liquid nitrogen, then add 700 μl of DNA extraction buffer, and bathe in water at 65° C. for 40 minutes. Add 700 μl of chloroform:isoamyl alcohol (24:1) and mix well. Centrifuge at 10,000 rpm for 5 minutes, and transfer the supernatant to a new centrifuge tube. Add 2 / 3 to 1 times the volume of pre-cooled isopropanol, and mix gently until the DNA precipitates. Centri...

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Abstract

The invention discloses a molecular marker linked with a paddy nitrogen nutrition regulation and control gene, which belongs to the technical field of agrobiology. The molecular marker respectively refers to an SSR molecular marker RM5897 and an STS molecular marker AP5756 positioned on a second chromosome of the paddy. The molecular marker linked with the paddy nitrogen nutrition regulation and control gene of the invention can be used for the identification of novel paddy nitrogen nutrition regulation and control products and the auxiliary selection breeding of the paddy with high nitrogen use efficiency, and the defects of high work load, high difficulty, easy influence of environment, long breeding period and the like of the conventional method can be overcome through the auxiliary selective breeding of the molecular marker. At the same time, the invention lays the foundation for cloning the nitrogen nutrition regulation and control genes. In addition, the invention has the certain practical meaning for selectively breeding environment protection novel paddy varieties, improving the fertilizer utilization rate and protecting the environment.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to molecular markers linked with rice nitrogen nutrition regulation genes and applications thereof. Background technique [0002] Nitrogen is the most important nutrient for rice growth. For half a century, with the cultivation and popularization of short-stalked, fertilizer-resistant and high-efficiency varieties, countries all over the world have regarded increasing nitrogen fertilizer as an important agricultural measure to increase rice yield. However, although the amount of chemical fertilizers has increased year by year in recent years, and its proportion in production costs has increased year by year, grain production has been stagnant. From 1984 to 1993, my country's chemical fertilizer consumption increased by 81%, while the total grain output only increased by 14.6%. Moreover, the problem of environmental pollution caused by high nitrogen is becoming...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 郭龙彪钱前马丽莲姜华曾大力高振宇
Owner CHINA NAT RICE RES INST
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