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A kind of vaccine for the prevention and/or treatment of dengue fever

A technology for dengue fever and dengue fever virus, applied in gene therapy, genetic material components, virus antigen components, etc., can solve the problems of reducing the safety of dengue fever vaccines, hindering the research and development of dengue fever vaccines, etc., and achieve the effect of reducing the virulence rate

Active Publication Date: 2016-11-30
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The antibody-dependent enhancement effect greatly reduces the safety of traditional dengue fever vaccines, seriously hindering the development of dengue fever vaccines

Method used

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  • A kind of vaccine for the prevention and/or treatment of dengue fever
  • A kind of vaccine for the prevention and/or treatment of dengue fever
  • A kind of vaccine for the prevention and/or treatment of dengue fever

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, the application of the substance that inhibits mosGCTL-3 gene expression in inhibiting dengue fever virus replication

[0029] The C-type lectin subtype 3 of Aedes aegypti is also called mosGCTL-3 protein, as shown in sequence 1 of the sequence listing. The gene encoding mosGCTL-3 protein is also called mosGCTL-3 gene, and the coding region of its cDNA is shown in sequence 2 of the sequence listing.

[0030] 1. Design dsRNA

[0031] The dsRNA (dsRNA-1) designed to inhibit the expression of the mosGCTL-3 gene is the double-stranded RNA corresponding to the double-stranded DNA shown in the 1st to 333rd nucleotides from the 5' end of sequence 2 in the sequence listing.

[0032] The dsRNA (dsRNA-2) designed to inhibit the expression of the GFP gene is the double-stranded RNA shown in sequence 19 of the sequence listing.

[0033] 2. Application of substances that inhibit the expression of mosGCTL-3 gene in inhibiting the replication of dengue fever type 2 vir...

Embodiment 2

[0046] Embodiment 2, the application of the substance that inhibits mosGCTL-26 gene expression in inhibiting dengue fever virus replication

[0047] The C-type lectin subtype 26 of Aedes aegypti is also called mosGCTL-26 protein, as shown in sequence 3 of the sequence listing. The gene encoding mosGCTL-26 protein is also called mosGCTL-26 gene, and the coding region of its cDNA is shown in sequence 4 of the sequence listing.

[0048] 1. Design dsRNA

[0049] The dsRNA (dsRNA-3) designed to inhibit the expression of the mosGCTL-26 gene is the double-stranded RNA corresponding to the double-stranded DNA shown in the 31-381 nucleotides from the 5' end of sequence 4 in the sequence listing.

[0050] 2. Application of the substance inhibiting the expression of mosGCTL-26 gene in inhibiting the replication of dengue fever type 2 virus

[0051] 1. Divide Aedes aegypti into two groups, and carry out the following treatments respectively:

[0052] Experimental group: each Aedes aegy...

Embodiment 3

[0063] Embodiment 3, the application of the substance that inhibits mosGCTL-15 gene expression in inhibiting dengue fever virus replication

[0064] The C-type lectin subtype 15 of Aedes aegypti is also called mosGCTL-15 protein, as shown in sequence 5 of the sequence listing. The gene encoding mosGCTL-15 protein is also called mosGCTL-15 gene, and the coding region of its cDNA is shown in sequence 6 of the sequence listing.

[0065] 1. Design dsRNA

[0066] The dsRNA (dsRNA-4) designed to inhibit the expression of the mosGCTL-15 gene is the double-stranded RNA corresponding to the double-stranded DNA shown in the 79th to 407th nucleotides from the 5' end of sequence 6 in the sequence listing.

[0067] 2. Application of substances that inhibit the expression of mosGCTL-15 gene in inhibiting the replication of dengue fever type 2 virus

[0068] 1. Divide Aedes aegypti into two groups, and carry out the following treatments respectively:

[0069] Experimental group: each Aedes ...

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Abstract

The invention discloses a vaccine for preventing and / or treating dengue fever. The present invention provides a product, the active ingredient of which is a substance that inhibits the expression of the gene encoding C-type lectin in Aedes mosquito; the use of the product is as follows (a) or (b) or (c): (a) Inhibit the replication of dengue virus in Aedes mosquito; (b) prevent and / or treat dengue fever; (c) block the transmission of dengue virus. The present invention finds that inhibiting the activity of Aedes C-type lectin protein can inhibit the replication of dengue fever virus in Aedes mosquitoes, and cut off the ability of Aedes mosquitoes to obtain dengue fever virus from the host body during the blood-sucking process, thereby significantly reducing the mosquito's infection rate, Reach the purpose of preventing and treating dengue fever virus. The invention has great value for the control of dengue fever, and has far-reaching significance for the cause of human health.

Description

technical field [0001] The present invention relates to a vaccine for preventing and / or treating dengue fever. Background technique [0002] Dengue fever (Dengue Fever, DF) is a viral acute infectious disease transmitted by Aedes mosquitoes. Dengue virus (DENV) is spherical and consists of cell membrane, capsid protein, nonstructural protein and single-stranded positive-strand RNA. In nature, there are four serotypes of dengue virus (DENV-1, DENV-2, DENV-3, and DENV-4). After the dengue virus infects the human body through the bite of an Aedes mosquito, it can show clinical symptoms after 2-3 days of incubation. The clinical features of dengue fever are high fever, bone and muscle pain, bleeding tendency and significantly decreased white blood cell count. The clinical manifestations of dengue fever are mild, and most people recover spontaneously with the progress of the disease, and the fatality rate is low. But the course of a few people will progress to the next stage, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/12A61K48/00A61P31/14
Inventor 程功刘洋
Owner TSINGHUA UNIV
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