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Nucleic acid membrane strips and kits for hbv typing and drug resistance mutation gene detection

A technology for drug resistance mutation and gene detection, which is applied to the determination/inspection of microorganisms, biochemical equipment and methods, etc. It can solve the problems of insufficient scientific accuracy, wrong detection and missed detection, and inability to detect recombinant types. Reliable results, comprehensive effect of mutation types

Active Publication Date: 2015-12-30
亚能生物技术(深圳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing typing detection products or patents, only a small number of probes (1 or 2) are used for typing, that is to say, only one or two sites in the genome are used for typing, which is not scientific and accurate enough , or cause false detection and missed detection, and cannot detect recombinant types

Method used

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  • Nucleic acid membrane strips and kits for hbv typing and drug resistance mutation gene detection
  • Nucleic acid membrane strips and kits for hbv typing and drug resistance mutation gene detection
  • Nucleic acid membrane strips and kits for hbv typing and drug resistance mutation gene detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Technical principles

[0032] 1. Design and implement primers and amplification reaction solutions based on known HBV genome research results.

[0033] According to the sequence characteristics of the HBV genome S region and reverse transcriptase region, PCR primers were designed to amplify the target fragments for typing and drug resistance mutation detection. In order to improve the hybridization efficiency between the PCR product and the probe, the PCR amplification product was designed within 300bp, so 4 pairs of primers were designed to amplify the genes of the S region in 4 segments for typing detection; 2 pairs of primers were designed to amplify in 2 segments The gene in the reverse transcriptase region is used for drug resistance mutation detection. In order to improve the amplification sensitivity, a pair of S region gene full-length amplification primers and 4 pairs of typing amplification primers were designed to form a nested 4-fold PCR in 1 tube of rea...

Embodiment 2

[0076] Embodiment 2 The use of kit of the present invention

[0077] 1. Intended use

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PUM

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Abstract

The invention relates to a disease pathogen gene detection technology, and particularly relates to a nucleic acid membrane strip and kit for HBV parting and drug resistance mutation gene detection. The nucleic acid membrane strip comprises a base, a nucleic acid probe fixed on the base, a G-membrane strip and an M-membrane strip; the G-membrane strip comprises nucleic acid probes for parting a hepatitis B virus and base sequences of the nucleic acid probes are as shown in SEQ ID NO:1-24; the M-membrane strip comprises nucleic acid probes for drug resistance mutation detection of the hepatitis B virus and the base sequences of the nucleic acid probes are as shown in SEQ ID NO:25-58. The nucleic acid membrane strip and kit for HBV parting and drug resistance mutation gene detection have the beneficial effect that HBV gene parting and drug resistance mutation detection can be comprehensively and quickly carried out.

Description

technical field [0001] The invention relates to a gene detection of a disease pathogen, in particular to a nucleic acid membrane strip and a kit for HBV typing and drug resistance mutation gene detection. Background technique [0002] Hepatitis B virus (HepatitisBVirus, HBV) belongs to hepadnaviridae, and its genome is about 3.2kb in length, which is partially double-stranded circular DNA. HBV infection mainly causes inflammatory lesions in the host liver, and can cause multiple organ damage, which is called viral hepatitis B, commonly known as hepatitis B. [0003] HBV is mainly transmitted through blood and blood products, mother to child, broken skin and mucous membranes, and sexual contact. HBV infection is prevalent worldwide, but the epidemic intensity of HBV infection varies greatly in different regions. According to the report of the World Health Organization, about 2 billion people in the world have been infected with HBV, of which 350 million people are chronic H...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/68
CPCC12Q1/706
Inventor 林斯里陈志强田洁
Owner 亚能生物技术(深圳)有限公司
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