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Preparation method of biological additive for improving effect of alfalfa green manure

A biological additive, alfalfa technology, applied in fertilizer mixtures, fertilization devices, applications, etc., can solve problems such as restricting the efficiency of green manure, slow decomposition of green manure, and increasing rice yield, etc., to increase phosphorus absorption, increase crop yield, and improve Effect of Phosphorus Content

Active Publication Date: 2014-12-10
SHANGHAI JIAO TONG UNIV SUBEI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Paddy fields have been flooded for a long time, and green manure decomposes relatively slowly. The release of organic phosphorus from green manure is the key to restricting the efficiency of green manure. At present, no technical measures have been found that can effectively promote the release of organic phosphorus from green manure in rice fields and increase rice yield.

Method used

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  • Preparation method of biological additive for improving effect of alfalfa green manure
  • Preparation method of biological additive for improving effect of alfalfa green manure
  • Preparation method of biological additive for improving effect of alfalfa green manure

Examples

Experimental program
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Effect test

Embodiment 1

[0032] 1) Preparation of microbial agents

[0033] (1) Inoculate and activate Bacillus megaterium and Bacillus cereus in MRS liquid medium, temperature 28°C, 125r.min -1 Shake culture for 48-72 hours to form the microbial agent, and the total number of Bacillus megaterium and Bacillus cereus contained in each ml of liquid medium exceeds 10 8 cfu;

[0034] 2) Preparation of alfalfa pulp syrup

[0035] (1) Take by weighing 300g fresh whole plant alfalfa raw material, chop into 3-5cm;

[0036] (2) the whole plant alfalfa raw material in the described (1) comprises branches and leaves and roots of alfalfa;

[0037] (3) by alfalfa raw material: sucrose=100g: the ratio of 5g takes by weighing sucrose or the syrup of equivalent sucrose amount;

[0038] (4) Measure clear water according to the ratio of alfalfa raw material (g) and water (ml) = 1:2;

[0039] (5) Fully mix the materials and substances weighed in (1), (3) and (4), mash them with a high-speed tissue grinder, let stan...

Embodiment 2

[0049] 1) Preparation of microbial agents

[0050] (1) Inoculate and activate Bacillus megaterium and Bacillus cereus in MRS liquid medium, temperature 30°C, 125r.min -1 Shake culture for 48-72 hours to form the microbial agent, and the total number of Bacillus megaterium and Bacillus cereus contained in each ml of liquid medium exceeds 10 8 cfu;

[0051] 2) Preparation of alfalfa pulp syrup

[0052] (1) Take by weighing 300g fresh whole plant alfalfa raw material, chop into 3-5cm;

[0053] (2) the whole plant alfalfa raw material in the described (1) comprises branches and leaves and roots of alfalfa;

[0054] (3) by alfalfa raw material: sucrose=100g: the ratio of 5g takes by weighing sucrose or the syrup of equivalent sucrose amount;

[0055] (4) Measure clear water according to the ratio of alfalfa raw material (g) and water (ml) = 1:2;

[0056] (5) Fully mix the materials and substances weighed in (1), (3) and (4), mash them with a high-speed tissue grinder, let stan...

Embodiment 3

[0067] 1) Preparation of microbial agents

[0068] (1) Inoculate and activate Bacillus megaterium and Bacillus cereus in MRS liquid medium, temperature 30°C, 125r.min -1 Shake culture for 48-72 hours to form the microbial agent, and the total number of Bacillus megaterium and Bacillus cereus contained in each ml of liquid medium exceeds 10 8 cfu;

[0069] 2) Preparation of alfalfa pulp syrup

[0070] (1) Take by weighing 300g fresh whole plant alfalfa raw material, chop into 3-5cm;

[0071] (2) the whole plant alfalfa raw material in the described (1) comprises branches and leaves and roots of alfalfa;

[0072] (3) by alfalfa raw material: sucrose=100g: the ratio of 5g takes by weighing sucrose or the syrup of equivalent sucrose amount;

[0073] (4) Measure clear water according to the ratio of alfalfa raw material (g) and water (ml) = 1:2;

[0074] (5) Fully mix the materials and substances weighed in (1), (3) and (4), mash them with a high-speed tissue grinder, let stan...

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Abstract

The invention provides a preparation method of a biological additive for improving the effect of an alfalfa green manure. The preparation method of the biological additive for improving the effect of the alfalfa green manure comprises the following steps: firstly preparing a microorganism bacterium agent and alfalfa pulp sugar liquid, then taking 10-30ml of a liquid culture medium in which the microorganism bacterium agent is preserved and 200-400ml of the alfalfa pulp sugar liquid, mixing and adding clear water till the volume is 1L, standing at 28-30 DEG C for 24 hours, filtering by using double-layer gauze and adding clear water to filtrate till the volume is 1L, thus obtaining the biological additive. The biological additive prepared by the method is capable of remarkably accelerating decomposition of the alfalfa green manure and soil organic phosphorus, increasing the content of soil rapidly available phosphorus in soil and improving phosphorus absorption of crops so as to improve the effect of the alfalfa green manure for increasing the crop yield.

Description

technical field [0001] The invention relates to a green manure synergist in the field of agricultural technology, in particular to a method for preparing a biological additive for alfalfa green manure synergist. Background technique [0002] Phosphorus plays an important role in promoting the growth and development of crops, and is one of the important nutrients for the growth and development of crops. However, most organic phosphorus and inorganic phosphorus in the soil cannot be directly absorbed and utilized by crops. 2+ , Fe 3+ 、Al 3+ Plasma combined to form insoluble phosphate, which is fixed in the soil, resulting in a decrease in the utilization efficiency of phosphorus in the soil and affecting crop yields. Green manure is an effective agronomic measure to improve farmland, increase the organic matter of farmland soil, reduce the use of chemical fertilizers, and increase crop yields. Today, with the increasing pollution of agricultural non-point sources, the use of...

Claims

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Application Information

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IPC IPC(8): C05G3/04C05G3/80
Inventor 安渊周鹏刘丹阳李强苏连泰吕爱敏张菁
Owner SHANGHAI JIAO TONG UNIV SUBEI RES INST