Use of odd-numbered and branched-chain fatty acids as markers for identifying animal species in milk
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A branched-chain fatty acid and animal milk technology, which is applied to measuring devices, instruments, scientific instruments, etc.
Active Publication Date: 2016-04-13
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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[0005] So far, there is no report on the application of OBCFA as a marker to identify animal species in milk
Method used
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experiment example 1
[0017] Experimental example 1 Establishment of the method for distinguishing cow milk, yak milk, buffalo milk, Jersey milk, horse milk, camel milk and goat milk according to the present invention
[0021] Dissolve 2g of sodium hydroxide in 100ml of methanol with a water content of no more than 0.5%. After the solution is left for a period of time, white precipitation of sodium carbonate may occur and become invalid. At this time, it is re-prepared. The solution was kept refrigerated.
[0023] Slowly add 10ml of acetyl chloride to 100ml of anhydrous methanol and store in refrigerator.
[0024] 1.1.3Na 2 SO 4 the solution
[0025] 6.67gNa 2 SO 4 Dissolve in 100ml pure water.
[0026] 1.1.4 Mixed solution of n-hexane / isopropanol
[0027] Mix n-hexane / isopropanol in a ratio of 3:2 and store in refrigerato...
experiment example 2
[0064] Experimental Example 2 The practical application experiment of using the method of the present invention to distinguish cow's milk, yak's milk, buffalo's milk, Jersey's milk, horse's milk, camel's milk and goat's milk
[0065] Collect 30 milk samples of cows (Beijing), 30 samples of goat milk (Hebei), 30 samples of buffalo milk (Yunnan), 30 samples of yak milk (Qinghai), 30 samples of Jersey milk (Hebei), camel milk 30 samples (Xinjiang) and 30 samples of horse milk (Xinjiang) were stored at -80°C until the samples were tested, and gas chromatography-mass spectrometry (GC-MS) was used to determine the following parameters of odd-numbered carbon-chain branched-chain fatty acids (OBCFA) in each sample: The content of the components: anteisoC13:0, isoC14:0, anteisoC15:0, isoC15:0, C15:0, isoC16:0, anteisoC17:0, isoC17:0 and C17:0.
[0066] Gas chromatography conditions and mass spectrometry conditions are the same as in Experimental Example 1.
[0067] The content of odd-...
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Abstract
The invention discloses uses of odd-number and branched aliphatic acids as animal species milk markers. A method includes: (1) acquiring data of the total content of the odd-number and branched aliphatic acids and the content of each component in each detection sample by utilization of a gas chromatography-mass spectrometry combined method; and (2) distinguishing each detection sample by analyzing the data of the total content of the odd-number and branched aliphatic acids and the content of each component. The data of the total content of the OBCFA and the content of each component in each detection sample is acquired by utilization of the GC-MS method. The data is subjected to main-component analysis, clustering analysis, and the like to accurately distinguish each detection sample. Cow milk, yak milk, buffalo milk, Jersey cattle milk, horse milk, camel milk and goat milk are distinguished accurately by the method. Fundamental information for practical application of the OBCFA in the milk and further research of milk fat is also provided.
Description
technical field [0001] The present invention relates to a method for distinguishing animal milk, in particular to a method for distinguishing cow milk, yak milk, buffalo milk, Jersey milk, horse milk, camel milk and goat milk by using GC-MS, belonging to animal species milk Identification field. Background technique [0002] Milk fat in animal milk is a high-quality natural fat. Milk fat is dispersed in milk in the form of globules or droplets. Sexual phospholipids, cholesterol, proteins and enzymes. The main components of milk fat are triglycerides, phospholipids and sterols; there are more than 400 kinds of fatty acids combined with triglycerides, and more than 20 kinds are common (JENSENRG.The composition of bovine milklipids:January1995toDecember2000[J]. ):295-350.). In addition to providing nutrition, milk fat also has many physiological activities, especially the medium and short-chain fatty acids and unsaturated fatty acids in milk fat are beneficial to human healt...
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Application Information
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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/86
Inventor 卜登攀马露王加启南雪梅陈静廷
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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