Optical projection tomography device and method based on camera array

Abstract

The invention provides an optical projection tomography device and a method based on a camera array. A light source array and the camera array are utilized to realize two-dimensional projection images of different exposures of an organism, and high-quality reconstruction of a three-dimensional image of a biological sample is realized through a high dynamic range imaging technique and a filter back projection algorithm. According to the optical projection tomography device and the method based on the camera array, an imaging module mainly comprises a scanning acquisition module and a data processing module, wherein the scanning acquisition module mainly comprises: (1) a light source module formed by the light source array, an optical lens, a beam expander and a polarizer; (2) a biological sample platform formed by a stepping motor rotating platform and a sample clamping device; (3) a two-dimensional projection image acquisition module formed by a microscope, the camera array, a polarization analyzer and a filter plate; the data processing module mainly comprises: (1) high dynamic range image synthesis; (2) rotary center position correction; (3) filter back projection three-dimensional reconstruction.

Description

technical field [0001] The invention belongs to the field of imaging technology, and in particular relates to an optical projection tomography device and method based on a camera array, which can be used for the evaluation of drug efficacy and gene expression of biological tissues, organs and embryos of 1 mm to 10 mm. Background technique [0002] Optical projection tomography (OPT) technology uses the characteristics of visible light to travel along a straight line in small-sized organisms. The emitted beam penetrates the sample to obtain a projection map for three-dimensional imaging of anatomical structures (transmission OPT), similar to X-ray CT. OPT imaging technology has micron-level spatial resolution, 1-10mm imaging field of view, micron-level spatial resolution, no radiation, and low cost. It provides a new high-resolution three-dimensional imaging for small-sized biological samples. effective means. However, OPT imaging still has problems such as unclear sample im...

AI Technical Summary

Problems solved by technology

[0004] However, due to the absorption heterogeneity of biological samples and the limitation of the dynamic range of the camera, the two-dimensional transmission images of biological samples formed by traditional optical projection tomography have different imaging effects for parts of the sample with different thicknesses, resulting in unsatisfactory reconstruction results. The details of the 3D image are not clear, and even some areas cannot be imaged

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