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Flos Caryophylli extractive and application thereof

A technology of male clove extract and male clove, applied in application, biocide, animal repellent, etc., can solve the problems of few sources and high acquisition cost, and achieve the effect of great application value

Inactive Publication Date: 2014-12-31
FUJIAN TOBACCO CORP NANPING CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with bio-control microorganisms, in addition to the advantages of natural pollution-free, plant-derived antibacterial substances have more stable preparation conditions and control effects, so they have important application prospects, but the above-mentioned plant-derived antibacterial substances have fewer sources and higher acquisition costs

Method used

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  • Flos Caryophylli extractive and application thereof
  • Flos Caryophylli extractive and application thereof
  • Flos Caryophylli extractive and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Screening of Chinese herbal medicines resistant to R. solanacearum

[0024] 1.1 Materials

[0025] The 40 kinds of Chinese medicinal materials purchased from Beijing Tongrentang Qingdao branch are shown in Table 1, and the medicinal materials resistant to R. solanacearum were screened out.

[0026] Tested strains: Pathogenic R. solanacearum was isolated and purified from infected strains collected from tobacco fields by Tobacco Research Institute, Chinese Academy of Agricultural Sciences.

[0027] Medium: beef extract peptone solid and liquid medium (NA medium), oat medium, potato dextrose agar medium (PDA medium)

[0028] 1.2 Preparation of medicinal material extract

[0029] The purchased 40 kinds of plants were dried in the shade, placed in a 50°C electric heating constant temperature blast drying oven, dried by a pulverizer, and passed through a 0.425mm sieve after pulverization. Weigh 50.0g of plant dry powder into a 500mL triangular flask, add 300mL o...

Embodiment 2

[0040] The virulence of embodiment 2 cloves to bacterial wilt

[0041] Test agent: male clove extract (extracted according to Example 1, and freeze-dried). According to the preliminary test results, the test agents were formulated into 5 kinds of concentration gradients with sterile water.

[0042] The toxicity of clove extract to R. solanacearum was determined by plate colony counting method. Bacteria solanacearum was cultivated on the slant medium for 40 hours, then added sterile water and scraped off the bacterial lawn to make a bacterial liquid, and after high-speed shaking for 120 seconds, diluted with sterile water to obtain about 50 bacterial cells / mL of the suspension; transfer 2 mL of the prepared dilution of the test agent into a sterile Petri dish, accurately add 1.0 mL of the bacterial suspension with a pipette, and then pour into the melted NA medium (liquid temperature 40°C ~50°C) 10 ml, vibrate the culture dish to fully mix the liquid medicine, bacteria and c...

Embodiment 3

[0048] Example 3 Potted Plant Test of Clove Extract Controlling Tobacco Bacterial Wilt

[0049] The male clove extract prepared in Example 1 (the following examples all adopt the male clove extract prepared in the manner of Example 1) to be concentrated to dryness, after dissolving with a very small amount of methanol, dilute with sterile water and settle to 100g / L, carry out the greenhouse disease control experiment and then dilute to the required concentration with sterile water. There are 5 treatments in the test, treatment 1: male clove extract 5g / L; treatment 2: male clove extract 2.5g / L; treatment 3: male clove extract 1.5g / L; treatment 4 (contrast 1): for Clear water, treatment 5 (control 2): ​​1 g / L streptomycin sulfate for agricultural use. 5 tobacco seedlings per treatment, repeated 3 times, randomly arranged.

[0050]Take tobacco seedlings with 5-6 true leaves and gently wash the roots, and transplant them into flowerpots. About 3 days after slowing down the see...

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Abstract

The invention discloses a Flos Caryophylli extractive, which is prepared by the following steps: (1) drying the Flos Caryophylli leaves at 40-65 DEG C, and crushing; (2) adding 95% ethanol according to the weight to volume ratio of 1: 3-9, conducting microwave treatment while electric heating at 500-600 W for 70-120 s, and filtering to obtain a filtrate; (3) adding 95% ethanol according to the weight to volume ratio of 1: 3-9, soaking 4-6 d, and filtering to obtain a filtrate; (4) adding 95% ethanol according to the weight to volume ratio of 1:3-9, conducting ultrasonic extraction with power of 500-650W for 1-1.5 h, and filtering to obtain a filtrate; and (5) merging the filtrates obtained in the step (2), (3) and (4), concentrating at 40-55 DEG C and 400-600 mm of mercury column to obtain an extract. The invention also discloses the application of the extractive to control of tobacco bacterial wilt disease. The invention can inhibit the growth of Pseudomonas solanacearum; after treatment by the extractive, Ralstonia solanacearum flagella disappear and the thalli are digested, so as to inhibit thalli protein synthesis, and reduce POD, CAT and SDH enzyme activity. In addition, control effect of tobacco bacterial wilt in greenhouse and field reach 71.5% and 59.7% respectively, therefore the invention has great application value.

Description

technical field [0001] The invention relates to the technical field of plant disease biological control, in particular to a clove extract and its application. Background technique [0002] Tobacco bacterial wilt is a typical soil-borne disease caused by Ralstonia solanacearum, and it is one of the main devastating diseases on tobacco. In recent years, affected by factors such as climate warming, soil acidification, and planting system (continuous cropping), the scope and degree of harm have increased year by year, and it has become the main restriction on the cultivation of flue-cured tobacco in Fujian, Hubei, Chongqing, Hunan, Yunnan, Sichuan and other tobacco areas. one of the factors. Since there are basically no high-quality and disease-resistant tobacco varieties available in production, the control of tobacco bacterial wilt is mainly based on the control of agricultural streptomycin sulfate, and the control effect is not ideal, and due to the long service life, the co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/28A01P3/00
Inventor 林勇孔凡玉徐茜戴培刚陈志厚张成省徐辰生夏艳冯超王静韩腾董瑜
Owner FUJIAN TOBACCO CORP NANPING CORP
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