A method for improving the preservation effect of embryogenic callus of Agapanthus
A technique for embryogenic callus and Agacia japonica is applied in the field of improving the preservation effect of A. japonica embryogenic callus, can solve problems such as insufficient production and scientific research, achieve remarkable preservation effect, improve viscosity and thermal conductivity, damage reduction effect
Inactive Publication Date: 2016-03-02
SHANGHAI JIAOTONG UNIV
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Problems solved by technology
Relevant research has established a cryopreservation system for embryogenic callus of Agapanthus alba, and the relative survival rate of cells after preservation has reached 56.94%, but it is still not enough to meet the needs of production and scientific research
Method used
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Embodiment
[0048] 1) The embryogenic callus of Agapanthus subcultured for 20 days was pre-cultured at 4°C for 2 days at low temperature on MS solid medium containing 0.5 mol / L sucrose;
[0049] 2) Transfer to the loading solution for soaking at room temperature for 60 minutes;
[0050] 3) Transfer to vitrification solution and dehydrate at 0°C for 40 minutes;
[0051] 4) Finally, store in liquid nitrogen at ultra-low temperature.
[0052] After step 3), without removing the vitrification solution, the embryogenic callus of Agapanthus lily soaked in the vitrification solution is directly placed in liquid nitrogen for cryopreservation.
[0053] According to the above steps, the embryogenic callus of Agapanthus was divided into experimental group and control group.
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The invention discloses a method for improving a preservation effect of agapanthus embryonic callus. According to the invention, the agapanthus embryonic callus is processed by adopting a vitrification solution which contains a carbon nanometer material so as to improve the preservation effect of the agapanthus embryonic callus. The method specifically comprises the steps of preculture, loading liquid treatment, vitrification solution treatment and liquid nitrogen preservation, wherein the vitrification solution contains 0.1-0.5 g / L of carbon nano tubes. The method disclosed by the invention optimizes the preservation effect of the agapanthus embryonic callus remarkably; as the carbon nano-tubes are added to serve as an allogenic material, the vitrification ultra-low temperature preservation of plants is facilitated.
Description
technical field [0001] The invention relates to the field of preservation of plants or parts thereof, in particular to a method for improving the preservation effect of embryogenic callus of Agapanthus lilifolia. Background technique [0002] Cryopreservation is a modern in vitro preservation technology for germplasm resources developed in the 1970s. Usually stored in liquid nitrogen, the substance metabolism and growth activities in the preserved material cells are almost completely stopped, and they are in a relatively stable biological state, achieving the purpose of long-term preservation of germplasm. Ultra-low temperature preservation is currently the only method that does not require continuous subculture. Medium and long-term preservation method. Vitrification cryopreservation is to place cells or tissues in a vitrification solution composed of a certain proportion of permeable and non-permeable protective agents, so that the material and its vitrification solution ...
Claims
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IPC IPC(8): A01N3/00A01H4/00
Inventor 张荻任丽陈冠群岳建华邹梦雯申晓辉
Owner SHANGHAI JIAOTONG UNIV