A Molecular Marker Related to Piglet Diarrhea and Its Application
A technology for piglet diarrhea and molecular markers, which is applied in the field of animal molecular genetic marker preparation, can solve the problem that molecular markers cannot satisfy disease resistance breeding, etc., and achieve the effects of improving the ability to resist diarrhea diseases and increasing the economic benefits of pig farms.
Active Publication Date: 2017-03-08
NORTHEAST AGRICULTURAL UNIVERSITY
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[0007] The purpose of the present invention is to provide a molecular marker related to piglet diarrhea and its detection method and application. The molecular marker can be used as the application of pig anti-diarrhea genetic improvement to solve the problem that the currently identified molecular markers cannot meet the needs of disease-resistant breeding. question
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specific Embodiment approach 1
[0023] Specific embodiment 1: A molecular marker related to piglet diarrhea according to this embodiment, the nucleotide sequence of the molecular marker is shown in Seq ID No: 1 in the sequence table.
specific Embodiment approach 2
[0024] Embodiment 2: This embodiment differs from Embodiment 1 in that: there is a base mutation at 514 bp of the nucleotide sequence of the molecular marker, and the base mutation is a mutation from A to G. Others are the same as in the first embodiment.
specific Embodiment approach 3
[0025] Specific embodiment three: a kind of method of adopting molecular marker to detect piglet diarrhea of the present embodiment, it is to carry out according to the following steps: extract genomic DNA from the pig blood to be detected, design primer, carry out PCR amplification, use to PCR product TaqI-PCR-RFLP genotyping.
[0026] In this embodiment, after genotyping by TaqI-PCR-RFLP, the AA type is separated, that is, the genotype with a low individual diarrhea rate, and the seeds are reserved for breeding.
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The invention relates to the technical field of preparation of animal molecular genetic markers, particularly to a molecular marker related to piglet diarrhea as well as a detection method and an application of the molecular marker, and aims to solve the problem that candidate genes identified at present cannot meet the requirement of breeding for disease resistance. A nucleotide sequence of the molecular genetic marker is represented by a sequence table Seq ID No:1; a method for detecting the piglet diarrhea susceptibility / resistance comprises the steps as follows: extracting genome DNA from to-be-detected piglet blood, designing a primer, performing PCR (polymerase chain reaction) amplification, and parting PCR products with a TaqI-PCR-RFLP genotype, so that the piglet diarrhea susceptibility / resistance is detected. The marker is applied to piglet marker-assisted selective breeding. According to the marker, CISH is selected to be used as a piglet diarrhea candidate gene, and screening of CISH promoter area genetic variation and research on characters affecting the piglet diarrhea are conducted, so that a new marker-assisted selection technology is expected to be established and applied to breeding of new anti-diarrhea strains (breeds) of piglets.
Description
technical field [0001] The invention belongs to the technical field of animal molecular genetic marker preparation, and in particular relates to a detection method and application of a molecular marker for predicting and identifying piglet diarrhea by using CISH gene. Background technique [0002] Piglet diarrhea is a common disease in pig production, and it is one of the main diseases plaguing the pig industry in the world. Piglets suffering from diarrhea have a low feed rate, reduced growth efficiency, and stagnant growth, causing huge economic losses to the pig industry. Although piglet diarrhea has been controlled to a certain extent by taking measures such as strengthening feeding management, improving environmental sanitation conditions, and applying drugs and vaccines for treatment and prevention, in the long run, using molecular genetics methods and techniques, breeding of piglets with resistant New disease-resistant strains (species) with diarrhea ability will be t...
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IPC IPC(8): C12N15/11C12Q1/68
Inventor 牛步月王希彪刘志然韩小飞杨秀芹
Owner NORTHEAST AGRICULTURAL UNIVERSITY