Method for simultaneously preparing high-purity podophyllotoxin and deoxidized podophyllotoxin from sabina vulgaris

Abstract

The invention discloses a method for simultaneously preparing high-purity podophyllotoxin and deoxidized podophyllotoxin from sabina vulgaris. The method takes a high-speed counter-current chromatography device as separating and purifying equipment and comprises the following steps of: preparing a solvent system which forms an immobile phase and a mobile phase, and pressing and suffusing the prepared immobile phase into a working drum of a main machine in an air pressure mode to rotate the main machine, wherein an upper phase is the immobile phase and a lower phase is the mobile phase; then pumping dissolved ethanol extraction extract of sabina vulgaris and the mobile phase at a preset flow rate, and respectively collecting and podophyllotoxin and deoxidized podophyllotoxin components (obtained through separation) from the mobile phase according to a detection spectrogram. The liquid-liquid partition chromatography separation and preparation method disclosed by the invention completely overcomes the defects of irreversible adsorption, contamination, denaturation and the like of a sample on a solid filler and is simple and convenient to operate, low in organic solvent consumption and high in separation efficiency; and the purity of the obtained product is higher than 98%.

Description

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AI Technical Summary

Benefits of technology

This technology allows for efficient isolating and separating highly pure materials from plant material that contains both glucosylceroline (GC) and phytolitholedicta (PE). These two molecules have unique structures but they may differ due to their chemical composition or physical characteristics such as size, shape, surface charge, etc., making them difficult to separate effectively without expensive equipment like liquid extraction techniques. By utilizing this technique, researches on these products could lead to better understanding how chemistry works together during various stages of production processes.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the quality and quantity of bergenisks found throughout North America through isolative distillations involving specific types of chantercytotaxes called cathaytestropes. These bergensys contain three main bioactive agents - phytate esters, α-,β-,γ-,δ-(Liu et al., 2004); they play important roles in nutritional support systems like nuts and vegetables. However, these materials cannot be separated effectively without expensive methods like columnchromatography which requires significant capital assets and operating expenses.