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Method for building bemisia tabaci populations which contain rickettisa bacteria and do not contain rickettisa bacteria

A technology of Bemisia tabaci and population, applied in the field of animal husbandry, etc., can solve the problems that antibiotics and injection methods cannot obtain stable corresponding populations, endosymbiotic bacteria cannot be cultured in vitro, etc.

Active Publication Date: 2015-01-21
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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Problems solved by technology

[0006] In order to solve the problem that the endosymbiotic bacteria cannot be cultured in vitro and cannot obtain stable corresponding populations with antibiotics and injection methods in the current insect endosymbiotic research, the purpose of the present invention is to establish a stable bacterium that contains Rickettisa bacteria and does not contain Rickettisa bacteria. Bemisia tabaci population method

Method used

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  • Method for building bemisia tabaci populations which contain rickettisa bacteria and do not contain rickettisa bacteria
  • Method for building bemisia tabaci populations which contain rickettisa bacteria and do not contain rickettisa bacteria
  • Method for building bemisia tabaci populations which contain rickettisa bacteria and do not contain rickettisa bacteria

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Embodiment 1

[0027] (1) Populations of Bemisia tabaci were collected from all over the country and cultivated in the laboratory, wherein the light conditions were 14 hours during the day: 10 hours at night, and the relative humidity was 75% to 85%.

[0028] (2) When the population size is relatively large, select a number of Bemisia tabaci and observe them under a microscope to identify males and females.

[0029] (3) Get the melon seedling (Cucumismelo) that has grown two true leaves, and use a miniature insect cage ( figure 1 ) clipped on the leaves of melon seedlings. Since the endosymbiotic bacteria in each whitefly are different, use a sucking tube to suck one male and female whitefly, and connect it to the lower insect room of the miniature insect cage, that is, the melon the back of the leaf.

[0030] (4) After three to four days, the bemisia tabaci in the miniature insect cages were taken out with a self-made insect extractor. Melons were grown in large pitcher cages ( figure 2...

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Abstract

The invention relates to building of insect infection endosymbiosis bacteria populations, in particular to a method for building bemisia tabaci populations which contain rickettisa bacteria and do not contain rickettisa bacteria. The method includes the following steps that the bemisia tabaci populations are collected and cultured in a laboratory; a plurality of bemisia tabaci are selected and observed under a microscope so that male and female bemisia tabaci can be determined; one male bemisia tabaci and one famele bemisia tabaci are selected by sucking and inoculated to the back of a muskmelon leaf; culturing is performed; deoxyribonucleic acid (DNA) of single bemisia tabaci is extracted, then the DNA of the bemisia tabaci is subjected to molecular identification by a polymerase chain reaction (PCR) method, and purification is performed to obtain stable populations. According to the technical scheme, living conditions of the bemisia tabaci under natural survival conditions are simulated to build the populations, change of biological characteristics of the bemisia tabaci is absent, and influence of the rickettisa bacteria on the bemisia tabaci can be correctly known.

Description

technical field [0001] The invention relates to the establishment of an insect-infecting type endosymbiotic bacteria population, in particular to a method for establishing a whitefly population containing Rickettisa bacteria and not containing Rickettisa bacteria. Background technique [0002] Bemisiatabaci Gennadaius belongs to Hemiptera Hmioptera, whitefly family Aleyrodiae, small whitefly belongs to Bamisia, widely distributed in more than 90 countries and regions on all continents except Antarctica, and its host range is wide, including vegetables More than 600 field and greenhouse crops including , cotton and ornamental plants. Bemisia tabaci mainly have the following three hazards. One is to feed on plant juice, causing its physiological abnormalities; the other is to secrete honeydew to induce sooty disease; . [0003] Endophytes are ubiquitous in nature, and almost all insects contain endosymbiotic bacteria. During long-term evolution, endosymbionts and their inse...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 杨义婷郭建洋万方浩刘万学刘怀
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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