Resistance evaluation method for stripe rust of wheat and method for controlling stripe rust of wheat by utilizing variety diversity

A technology of wheat stripe rust and evaluation method, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of wheat stripe rust retention, etc., and achieve the goal of ensuring correlation and improving accuracy Effect

Active Publication Date: 2019-05-10
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, for the diversity control of wheat stripe rust, there is still no complete and effective technical plan, and the diversity control of wheat stripe rust still stays at the research level of small-scale planting. Therefore, the development of effective utilization The method of controlling stripe rust by planting diverse varieties of wheat is of great significance to the ecological prevention and control of wheat stripe rust and the safety of wheat production

Method used

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  • Resistance evaluation method for stripe rust of wheat and method for controlling stripe rust of wheat by utilizing variety diversity
  • Resistance evaluation method for stripe rust of wheat and method for controlling stripe rust of wheat by utilizing variety diversity
  • Resistance evaluation method for stripe rust of wheat and method for controlling stripe rust of wheat by utilizing variety diversity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Example 1 Method for evaluating stripe rust resistance of wheat varieties using molecular disease index and molecular relative parasitic fitness

[0079] This example provides a method for evaluating the resistance of wheat stripe rust. By artificially inoculating the wheat to be tested with stripe rust, extracting the DNA of wheat leaves during the incubation period, quantifying the stripe rust DNA and wheat DNA respectively, and calculating the stripe rust The ratio of DNA to wheat DNA is used to determine the stripe rust resistance level of the tested wheat according to the ratio of stripe rust DNA to wheat DNA. Taking the resistance evaluation of 100 wheat varieties selected from the popularized varieties and reserve varieties of Sichuan Province to the physiological races of stripe rust popular in Sichuan: stripe rust CYR32, CYR33 and pathogenic type G22-83 as an example, in In 2014-2015 and 2016-2017, two resistance evaluation experiments were carried out in Kaife...

Embodiment 2

[0100] Embodiment 2 adopts traditional disease investigation method to carry out resistance evaluation

[0101] The present embodiment provides a kind of resistance evaluation method of wheat stripe rust, for adopting traditional disease investigation method to carry out resistance evaluation, the sowing of wheat variety, indoor breeding and artificial inoculation of stripe rust in the field are the same as step 1, step 1 of embodiment 1. Described in 2 and 3, different from embodiment is not to carry out step 4,5 and 6 of embodiment 1, carry out following condition investigation after step 3 (field artificial inoculation stripe rust):

[0102] Disease investigation: The disease investigation started after Mingxian 169 showed symptoms. There were 5 investigations in total, with an interval of 3-5 days between each time. April 7 and April 1, 2017. A total of 100 wheat leaves from 25 plants were randomly selected for each variety to record the severity of the disease. Severity...

experiment example 1

[0108] Experimental example 1 Comparison of different resistance evaluation methods for the evaluation of wheat resistance level

[0109] Data Analysis: In order to compare with the traditional disease investigation method used in Example 2 to carry out resistance evaluation, analyze the molecular disease index MDI and molecular relative parasitic fitness (MRPF) of Example 1 to carry out wheat variety resistance evaluation In the two experiments of 2014-2015 and 2016-2017, the MDI calculated by collecting wheat leaves 14 days after artificial inoculation (MDI-14DAI) and the MDI calculated by collecting wheat leaves 15 days after artificial inoculation (MDI- 15DAI) were multiplied by a coefficient (Adjust-MDI-2014, Adjust-MDI-2016), so that the maximum value was the same as the AUDPC-DI (AUDPC-DI-2014, AUDPC-DI-2014, AUDPC-DI-2016) have the same maximum value to facilitate cluster analysis. Cluster analysis was performed on AUDPC-DI-2014, AUDPC-DI-2016, Adjust-MDI-2014 and Adj...

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Abstract

The invention relates to the technical field of crop planting, in particular to a resistance evaluation method for stripe rust of wheat and a method for controlling the stripe rust of the wheat by utilizing variety diversity. Puccinia striiformis is manually inoculated to to-be-detected wheat, wheat leaf DNA in the incubation period is extracted, puccinia striiformis DNA and wheat DNA are separately quantified, and the resistance level of the stripe rust of the to-be-detected wheat is judged according to the content ratio of the puccinia striiformis DNA in the wheat leaf DNA to the wheat DNA.Different resistance varieties are selected for mixed variety sowing according to indexes of the resistance level of the stripe rust of wheat varieties and the like, the wheat variety diversity planting mode is formed, the stripe rust states of the wheat and diseases are significantly reduced, and the stripe rust of the wheat can be efficiently controlled; the yield of wheat is effectively increased, service life periods of varieties are prolonged, the method is more beneficial for the stable population genetic structure of the puccinia striiformis, and important significance on ecological prevention and control for the stripe rust of the wheat is achieved.

Description

technical field [0001] The invention relates to the technical field of crop planting, in particular to a method for evaluating resistance to wheat stripe rust and a method for controlling wheat stripe rust by utilizing variety diversity. Background technique [0002] Wheat stripe rust is an important airborne fungal disease of leaves caused by the infection of Puccinia striiformis f.sp. Tririci. Repeated infestation of wheat within a growing season causes damage. [0003] Wheat stripe rust occurs throughout the main wheat planting areas, and has the characteristics of large occurrence area and serious yield loss, which seriously threatens the production safety of wheat. [0004] At present, planting resistant varieties is still the safest, economical and effective means to control wheat stripe rust. However, most resistant varieties lose their resistance after 3-5 years of large-scale application, and the main reason for the loss of variety resistance is the generation and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
Inventor 马占鸿初炳瑶黄冲孙振宇赵磊胥岩
Owner CHINA AGRI UNIV
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