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Method for Large-scale Creation of Rapeseed Rapeseed Genetically Stable Population by Double Haploid Inducer Line

A double-haploid, genetically stable technology, applied in biochemical equipment and methods, horticultural methods, and botanical equipment and methods, can solve problems such as low induction efficiency, and achieve the effect of promoting QTL analysis, reducing cycle and manpower

Active Publication Date: 2018-06-08
CHENGDU ACAD OF AGRI & FORESTRY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The earliest maize haploid induction line is stock6, which can only induce haploids in maize, and then the haploid plants undergo artificial chromosome doubling to form homozygous diploids (double haploids), and the induction efficiency Low, generally the induction efficiency is below 10% (calculated based on the number of haploids obtained from harvested seeds)

Method used

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  • Method for Large-scale Creation of Rapeseed Rapeseed Genetically Stable Population by Double Haploid Inducer Line
  • Method for Large-scale Creation of Rapeseed Rapeseed Genetically Stable Population by Double Haploid Inducer Line
  • Method for Large-scale Creation of Rapeseed Rapeseed Genetically Stable Population by Double Haploid Inducer Line

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Experimental program
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Effect test

Embodiment 1

[0080] see figure 1 , figure 2 , Figure 5 , Figure 7 , in order to study the high oil content genes and formation mechanism of Brassica napus, it is necessary to construct a DH stable genetic mapping population with more than 500 strains. F 1 , F 1 The plants were chemically killed, and in F 1 In the later stage of plant seedlings and early budding (middle and late December), spray the plants twice with tribenuron-methyl 80ppm, and artificially pollinate the double haploid induction line Y3380 obtained by the applicant in February-March of the next year to obtain a large number of induced offspring seeds. f 2 The offspring (induced offspring) were planted and tested by flow cytometry, and the selection was normal, the ploidy (tetraploid) was normal, and there was no dominant trait of the induced line (dwarf). f 3 The purity of the strains was identified, and 648 stable strains were obtained, and the near-infrared quality test was carried out on the oil content of ...

Embodiment 2

[0158] see figure 1 , figure 2 , Figure 4 , Figure 8 , in order to study the early maturity traits of Brassica napus, it is necessary to construct a DH stable genetic mapping population with more than 1000 strain populations. F 1 ,F 1 Plants were chemically killed, and in F 1 In the later stage of plant seedlings and early budding (middle and late December), the plants were sprayed twice with 100ppm of pentoxychlor, and planted in an isolation net room with the rapeseed double haploid induction line Y3560 obtained by the applicant. At the flowering stage in early March, a large number of induced progeny seeds were obtained by assisted pollination by the bee. f 2 The offspring (induced offspring) were planted and tested by flow cytometry, and the selection was normal, the ploidy (tetraploid) was normal, and there was no dominant trait of the induced line (dwarf). f 3 The purity of the generation strains was identified, and 1545 stable strains were obtained, and the...

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Abstract

The invention provides a method for creating a rape genetic-stability group through a rape doubled haploid inducible system in large scale. The method comprises the steps: 1), according to research requirements, selecting and breeding an objective trait; 2), according to the objective trait, selecting and breeding two parent materials with relatively large objective trait difference; 3), enabling the two parent materials to be hybridized; 4), pollinating parent hybridized F1 generation by using the rape doubled haploid inducible system; 5), discriminating induced progeny genetic stability; 6), performing induced progeny objective trait research to form the genetic-stability group. The method provided by the invention can be applied to three cultivated species of rapes, including cabbage type rapes (2n=38), turnip type rapes (2n=20) and mustard type rapes (2n=36), can obtain rapid (three-generation) large-scale genetic-stability DH (double haploid) groups, has a remarkable facilitation function to rape fundamental research, particularly application of genetic mapping groups to gene mapping, gene fine mapping and QTL analysis, shortens the cycle of rape fundamental research, and reduces the labor and material investments.

Description

Technical field: [0001] The present invention is related to agriculture, in particular to a method for large-scale creation of rapeseed genetically stable populations, ie DH populations, of rapeseed double haploid induction lines. Background technique: [0002] Rapeseed is the main oil crop in my country, including 3 cultivars, Brassica napus ( Brassica napus , a compound species evolved from Brassica oleracea (aa, n=10) and Brassica cabbage (cc, n=9) through natural interspecific hybridization and double diploidization. According to the source of chromosomes, it is tetraploid, 2n=38 ); Brassica napus ( Brassia campestris L. Including Brassica and Chinese cabbage native to China. China is also known as Chinese cabbage, dwarf rape, sweet rape and so on. The chromosome group is aa, n=10, and it is judged to be diploid according to the source of the source chromosome group, 2n=20); mustard type rape (Brassica juncea, composed of Brassica juncea (aa, n=10) and black mustard ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04A01H1/08A01H4/00C12Q1/68
CPCA01H1/02A01H1/04A01H1/08A01H4/001A01H4/008C12Q1/6895C12Q2600/156
Inventor 付绍红杨进王继胜李云邹琼陶兰蓉康泽明唐蓉殷丽琴
Owner CHENGDU ACAD OF AGRI & FORESTRY SCI
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