SSR molecular mark for identification of self-incompatibility of brassica campestris ssp. chinensis Makino, and application thereof

A head cabbage and molecular marker technology, applied in the fields of crop genetics, breeding and biology, can solve the problems of large workload, long detection period, easy to be restricted by environmental conditions, etc., and achieve the effect of improving reliability and rapid typing

Active Publication Date: 2015-01-21
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to aim at the defects of the affinity index method in non-heading Chinese cabbage breeding, such as long detection period, heavy workload, easy to be limited by environmental conditions, etc., to provide a method that can self-seed non-heading Chinese cabbage at the seedling stage. SSR molecular markers for affinity detection, so as to quickly identify the self-incompatibility of non-heading Chinese cabbage plants, and speed up the process of molecular marker-assisted breeding of non-heading Chinese cabbage

Method used

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  • SSR molecular mark for identification of self-incompatibility of brassica campestris ssp. chinensis Makino, and application thereof
  • SSR molecular mark for identification of self-incompatibility of brassica campestris ssp. chinensis Makino, and application thereof
  • SSR molecular mark for identification of self-incompatibility of brassica campestris ssp. chinensis Makino, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Detection of self-incompatibility of non-heading Chinese cabbage parents and F2 generation individual plants

[0046] The specific implementation steps of this example are:

[0047] 1. Establishment of parental materials and hybrid populations:

[0048] The parents are two near-isogenic lines of non-heading Chinese cabbage variety 'Aijiaohuang': self-compatible line 002 and self-incompatible line 210, bred and preserved by the cabbage research group of Yu Horticultural College of Nanjing Agricultural University (Cao Shouchun , Hou Xilin, Hao Xiuming. Research on Breeding and Seed Propagation Techniques of Non-heading Chinese Cabbage Bantamia incompatibility Line. Journal of Nanjing Agricultural University, 2002, 25: 111-113). Among them, 002 showed strong self-incompatibility, and the affinity index was less than 1.0; 210 showed self-compatibility, and the affinity index was more than 5.0. Using 210 as the female parent and 002 as the male parent for cross-...

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Abstract

The invention discloses an SSR molecular mark for identification of self-incompatibility of brassica campestris ssp. chinensis Makino, and an application thereof. A mark primer is at least one selected from Na12E02, KBRH138G23 or BrSS15. The SSR molecular mark provided by the invention can be applied in rapid identification and detection of the self-incompatibility of brassica campestris ssp. chinensis Makino, increase breeding efficiency and accelerate a breeding process.

Description

technical field [0001] The invention belongs to the fields of crop genetics and breeding and biotechnology, and in particular relates to SSR molecular markers linked to non-heading Chinese cabbage self-incompatibility genes and their application. Background technique [0002] Non-heading Chinese cabbage (Brassica campestris ssp.chinensis Makino), commonly known as green cabbage and Chinese cabbage, belongs to Brassica species of Brassica genus Brassica, and is the main leafy vegetable in my country. It has the characteristics of strong adaptability, short growth period, tender quality, unique flavor, and rich nutrition. It is a popular vegetable that people in southern my country like to eat all year round, and plays an important role in the annual supply of vegetables. The annual planting area is about 8 million mu, accounting for 30-40% of the multi-cropping area of ​​vegetables in the middle and lower reaches of the Yangtze River. In recent years, it has been widely intr...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 李英葛婷婷侯喜林王立
Owner NANJING AGRICULTURAL UNIVERSITY
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