Method for detecting byproducts 4-methylimidazole and 2-acetyl-4-hydroxy-butylimidazole in caramel pigment

A technology of methylimidazole and butylimidazole, which is applied in the inspection field of food safety inspection, can solve problems such as complex pretreatment technology and unfavorable sample analysis, and achieve effective separation and purification, and accurate and reliable results

Inactive Publication Date: 2015-01-21
JIANGSU PROVINCIAL CENT FOR DISEASE PREVENTION & CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, most of the first few techniques require more complex pretreatment techniques, which is very unfavorable for the analysis of a large number of samples.

Method used

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  • Method for detecting byproducts 4-methylimidazole and 2-acetyl-4-hydroxy-butylimidazole in caramel pigment
  • Method for detecting byproducts 4-methylimidazole and 2-acetyl-4-hydroxy-butylimidazole in caramel pigment
  • Method for detecting byproducts 4-methylimidazole and 2-acetyl-4-hydroxy-butylimidazole in caramel pigment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0066] (1) The preparation of the sample solution to be tested comprises the following specific steps:

[0067] (a) Preparation of solid-phase extraction sample solution: Weigh 1 g (accurate to 0.001 g) of the caramel pigment sample, place it in a 100 mL volumetric flask, and add water to make up to the mark. Take 0.1mL of the above sample solution, add 0.1mL hydrochloric acid solution (0.02moL / L), add 0.2mL internal standard solution (D6-4-MEI, 1.0μg / mL), add water to 2mL, mix well and wait for the solid phase Extraction and purification.

[0068] (b) Solid-phase extraction column activation: choose a mixed-type cation-exchange solid-phase extraction column, the matrix is ​​polystyrene-divinylbenzene polymer, 60mg, 3mL, or equivalent. Activate with 2mL methanol and 2mL water successively before use.

[0069] (c) Solid-phase extraction: Add the sample solution prepared in (a) into the freshly activated small column in (b), control the flow rate to be less than 0.5mL / min, dra...

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Abstract

A method for detecting byproducts 4-methylimidazole and 2-acetyl-4-hydroxy-butylimidazole in a caramel pigment comprises the following steps: 1, preparing a solution of a sample to be detected; 2, detecting the sample; and 3, analyzing result. The method has the advantages of rapid detection, high sensitivity, high accuracy and the like.

Description

technical field [0001] The invention relates to a detection method of by-products 4-methylimidazole and 2-acetyl-4-hydroxy-butylimidazole in caramel color, which belongs to the detection method of food safety detection. Background technique [0002] 4-Methylimidazole (4-MEI) is a by-product of the Maillard reaction (Maillard browning model systems) in the production and processing of Class III and IV caramel colors. Studies have shown that it can induce tumors . The European Food Safety Authority (EFSA) reported that Class III caramel color will also produce 2-acetyl-4-hydroxy-butylimidazole (2-acetyl-4-tetrahydroxy-butylimidazole, THI) during the production process. Immunotoxic. [0003] At present, for the determination of 4-MEI content in caramel pigment, my country only has the national standard GB8817-2001 for food additive caramel color, and there is no national standard for the determination of THI in caramel pigment. Internationally, 4-MEI and THI in the caramel c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 荣维广马永建吉文亮刘华良
Owner JIANGSU PROVINCIAL CENT FOR DISEASE PREVENTION & CONTROL
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