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Cultivation method of stem tissues of shiny-leaved yellowhorn

A cultivation method, the technology of X. sorbifolium, which is applied in the field of plant tissue cultivation, can solve the problems of strong seed variability, possibility of weakening, low seed setting rate of X. sorbifolium, etc., and achieve the effect of high survival rate and fast tissue differentiation speed

Inactive Publication Date: 2015-02-04
张勇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the seed embryo is a good experimental material and is easy to obtain, the low seed setting rate of X. sorbifolium and the good use value of the seeds all limit the way to use the seed embryo for propagation.
The most important thing is that the seeds of X. sorbifolium have strong variability, and may not be able to completely retain the excellent traits of excellent trees, which also greatly weakens the possibility of obtaining excellent seedlings through somatic embryogenesis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for cultivating stem tissue of sorbifolium sorbifolium provided by the invention, the method comprises the following steps successively:

[0023] 1) Disinfection treatment

[0024] Collect the middle and lower stems of the dormant branches of Xanthos sorbifolium in the current year, trim them, subtract the dead branches and air-dried branches, clean them with sterile water, put them in a wide-mouthed bottle, drip 75% hydrogen peroxide on the leaf axils, and then use 75 % alcohol disinfection for 25s, rinsed, then disinfected with bromogeramine for 30-60s, and washed with sterile water;

[0025] 2) Primary culture

[0026] Remove the top and bottom of the clean branches after disinfection, cut the stem section with a 4-6cm length and one axillary bud obliquely into the primary culture medium at 24-26°C, cultivate in light for 12 / 12h, and incubate at a light intensity of 1000-1500lux for 15-17 sky;

[0027] Wherein: the first-generation medium is prepared by t...

Embodiment 2

[0034] Another method for cultivating the stem tissue of sorbifolium sorbifolium provided by the present invention, the method comprises the following steps successively:

[0035] 1) Disinfection treatment

[0036] Collect the middle and lower stems of the dormant branches of Xanthos sorbifolium in the current year, trim them, subtract the dead branches and air-dried branches, clean them with sterile water, put them in a wide-mouthed bottle, drip 75% hydrogen peroxide on the leaf axils, and then use 75 % alcohol disinfection for 20-40s, rinsed, then disinfected with bromogeramine for 60s, and washed with sterile water;

[0037] 2) Primary culture

[0038] Remove the top and bottom of the clean branch after disinfection, cut the stem section with a length of 4-6cm, and insert an axillary bud obliquely into the primary culture medium at 24°C, light culture for 12 / 12h, and light intensity 1000-1500lux for 15-17 days;

[0039] Wherein: the first-generation medium is prepared by ...

Embodiment 3

[0046] Another method for cultivating the stem tissue of sorbifolium sorbifolium provided by the present invention, the method comprises the following steps successively:

[0047] 1) Disinfection treatment

[0048] Collect the middle and lower stems of the dormant branches of Xanthos sorbifolium in the current year, trim them, subtract the dead branches and air-dried branches, clean them with sterile water, put them in a wide-mouthed bottle, drip 75% hydrogen peroxide on the leaf axils, and then use 75 % alcohol disinfection for 20s, rinsed, then disinfected with bromogeramine for 60s, and washed with sterile water;

[0049] 2) Primary culture

[0050] Remove the top and bottom of the clean branch after disinfection, cut the stem segment with a length of 6cm, and insert an axillary bud obliquely into the primary culture medium at 26°C, cultivate in light for 12 / 12h, and cultivate for 17 days with a light intensity of 1000-1500lux;

[0051]Wherein: the first-generation medium...

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Abstract

The invention discloses a cultivation method of stem tissues of shiny-leaved yellowhorn. According to the main technical points of the invention, the method sequentially comprises the following steps: 1) carrying out disinfection treatment on the stem tissues; 2) primary culture, namely removing the top end and bottom end of a disinfected clean branch, cutting the branch into a 4-6 cm stem with an axillary bud, obliquely inserting the stem into a primary culture medium to carry out light culture for 12 / 12 h at a temperature of 24-26 DEG C, and culturing for 15-17 days at a light intensity of 1000-1500 lux; 3) subculture, namely cutting off an induced adventitious bud, grafting the stem subjected to bud cutting-off to a newly prepared primary culture medium to continue to culture, taking a bud every 15-17 days, and repeating for 3 times; and 4) rooting culture, namely grafting the adventitious buds to a rooting culture medium to carry out light culture for 12 / 12 h at a temperature of 24-26 DEG C, and culturing for 5-7 day at a light intensity of 1000-1500 lux. The method belongs to the technical field of plant tissue cultivation.

Description

technical field [0001] The invention discloses a method for cultivating plant tissues, in particular to a method for cultivating stem tissue of sorbifolium sorbifolium, belonging to the technical field of plant tissue cultivation. Background technique [0002] Xingguan fruit, also known as papaya and Wenguan fruit, is a high-grade oil plant. In the past, people did not pay attention to its fresh food, processing, medicinal and ornamental values. The sorbetia sorbifolium tree is graceful, with beautiful leaf shape, magnificent flower color, and late and long flowering period. In recent years, it has been cultivated as a new type of ornamental tree. Xantho sorbifolium has the characteristics of beautiful flowers, strange leaves, fruity fragrance, thin branches, and clumsy body. It is easy to artificially control the tree shape and create various bonsai. Highways, city streets, and scenic spots in northern areas can be used as ornamental tree cultivation. The wood is flesh r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张勇
Owner 张勇