A method for separating and enriching peony anthocyanins in a two-phase system

Abstract

The invention belongs to the field of separation and purification of natural product effective components, and particularly discloses a method for separating and enriching peony anthocyanin by using a double-water-phase system. The method comprises the following steps: drying and pulverizing fresh peony flowers at low temperature, adding a double-water-phase system composed of ethanol and ammonium sulfate, adding sucrose and organic acid as stabilizers of anthocyanin, magnetically stirring under ice water bath conditions, centrifugating to obtain an upper phase, a lower phase and peony residues, extracting the residues and lower phase many times, merging with the upper phase, carrying out impurity removal by alcohol precipitation and extraction, and carrying out vacuum concentration to obtain the peony anthocyanin. By adopting the double-water-phase system, the method has the advantages of mild conditions, short extraction time, recoverable organic phase and low extraction cost, and can easily implement industrialized scale-up production.

Description

[0001] (1) Technical field [0002] The invention belongs to the field of separation and purification of effective components of natural products, in particular to a method for separating and enriching peony anthocyanins in a two-phase system. [0003] (2) Background technology [0004] Peony (Paeoniasuffruticosa Andr), also known as deer leek, Atractylodes macrocephala, and wood peony, is a perennial deciduous shrub of the family Paeoniaceae and the genus Paeoniae. Heze City is a famous hometown of peony, and it is the largest peony cultivation center and germplasm resource center in the country and even in the world, with a cultivation area of ​​8,000 hectares. With the rapid development of the peony industry, it has played a certain role in promoting the tourism and economic and trade industries in the flower-growing areas. However, due to the restrictions of the flowering period and climatic conditions, the economic, trade and tourism activities are prosperous when the flow...

AI Technical Summary

Problems solved by technology

[0006] Anthocyanins are easily soluble in solvents such as water, methanol, and ethanol. Traditional extraction methods include water extraction, organic solvent extraction, or assisted extraction by ultrasonic, microwave, and enzymatic methods, but these methods require a large amount of solvent and a long extraction time , the equipment requirements are high, and there are many impurities in the extract, which needs further separation and purification, which is not conducive to industrial scale-up production. In addition, the stability of anthocyanins is poor, and the extraction process is easily affected by external factors such as temperature, pH, and metal ions. Browning or degradation, resulting in relatively low extraction yields

[0007] Aqueous two-phase extraction technology began in the 1960s. Traditional two-phase aqueous phases include dual polymer systems, polymer / glucose systems, polymer / inorganic salt systems, etc. However, due to high polymer prices, high viscosity, and recycling Difficult to adapt to large-scale industrial production. In recent years, small molecule hydrophilic alcohol / salt two-phase extraction has become a research hotspot in biological separation and purification. Compared with traditional polymer two-phase extraction, it has fast phase separation, It has the advantages of good effect, easy recovery of organic phase, and low cost. In addition, the condition of two-phase extraction is mild, which is beneficial to the structural stability of anthocyanins during the separation process.

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