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Enteromorpha prolifera high-valued extraction method

An extraction method and a high-value technology, applied in applications, biofuels, fertilization devices, etc., can solve the problem that it is difficult to completely crack the cell wall of plants, so as to enhance the activity and function of bacteria, improve the extraction rate, and speed up the process of plant growth. The effect of growth

Active Publication Date: 2015-02-11
邳州德好科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the extraction of sodium alginate by enzymatic extraction is not mature enough, and only a single cellulase is used for hydrolysis, but the plant cell wall consists of cellulase, hemicellulase, pectin, lignin and other components. The link between them is either mosaic or cohesion, so it is difficult to completely break the cell wall of the plant with a single enzyme, and the contents cannot be completely released

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Weigh 100 kg of dried and crushed Enteromorpha enteromorpha, add fresh drinking water with a raw material weight of 600 kg, soak in a water bath at 50°C for 2 hours, adjust the pH value of the liquid to 4 with HCl, add 45% endo-β-glucanase, exogenous Cut β-glucanase 30%, β-glucanase 25% cellulase 0.3%, enzymolysis 6h; add protease 0.2% neutral protease 45%, papain 55%, pectin Pectinase 0.2% composed of 55% lyase and 45% pectinesterase, continued enzymolysis for 3 hours, stirred, and centrifuged after enzymolysis;

[0026] Add 800kg of 2% NaHCO to the Enteromorpha after the above centrifugation 3 Digest the mixed solution with 1% sodium hydroxide in a water bath at 50°C for 2 hours; then add 5 times the volume of fresh drinking water at 50°C to dilute for 1 hour, after fully diluting evenly, filter it through a 200-mesh silk screen; add 0.3% of the filtrate Hydrogen peroxide, decolorize at 50°C for 1 hour, then raise the temperature to 75°C and keep for 10 minutes, fi...

Embodiment 2

[0030] Weigh 100 kg of dried and pulverized Enteromorpha enteromorpha, add fresh drinking water with a raw material weight of 900 kg, soak in a water bath at 60°C for 3 hours, adjust the pH value of the liquid to 7 with HCl, add 45% endo-β-glucanase, exogenous Cut β-glucanase 30%, β-glucanase 25% cellulase 0.5%, enzymolysis 8h; add protease 0.4% neutral protease 45%, papain 55%, pectin Pectinase 0.3% composed of 55% lyase and 45% pectinesterase, continued enzymolysis for 4 hours, stirred, centrifuged after enzymolysis;

[0031]Add 1000kg of 3% NaHCO to the Enteromorpha after the above centrifugation 3 Digest the mixed solution with 1.5% sodium hydroxide in a water bath at 60°C for 2.5h; then add 6000kg of fresh drinking water at 60°C to dilute for 2h, fully dilute and filter through a 300 mesh silk screen; Hydrogen peroxide, decolorize at 60°C for 2 hours, then raise the temperature to 85°C for 20 minutes, filter with 300-mesh silk screen; slowly add CaCl with a volume ratio...

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PUM

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Abstract

The invention discloses an enteromorpha prolifera high-valued extraction method, and is characterized in that the method comprises the following steps: (1) raw material pretreatment, (2) cellulose enzymolysis, (3) proteolysis, (4) brine digestion, (5) dilution and filtration, (6) decoloring, (7) CaCl2 solution treatment, (8) ion exchange, (9) preparation of sodium alginate, (10) preparation of ethanol, and (11) preparation of an enteromorpha prolifera organic fertilizer. A composite enzyme is prepared from protease, pectinase and cellulase by a scientific ratio, and raw material is pretreated, so as to help to improve the extraction rate of sodium alginate; at the same time, fuel ethanol is prepared by using cellulose-rich enteromorpha prolifera processing waste; the enteromorpha prolifera organic fertilizer is prepared through concentration, spray drying and granulation, so as to realize the high-valued comprehensive utilization of enteromorpha prolifera, and develop marine biological energy.

Description

technical field [0001] The invention relates to the field of utilization of Enteromorpha, in particular to a high-value extraction method of Enteromorpha. Background technique [0002] At present, the world is facing the huge pressure of energy shortage and environmental pollution, and the development of clean and renewable energy has become an important means to solve the crisis of petrochemical resources and energy. Bioethanol has been widely concerned as a clean fuel and gasoline additive. At present, the preparation of fuel ethanol in my country mainly focuses on terrestrial raw materials such as straw, bagasse, wood chips and cassava, but there are few studies on the preparation of bioethanol from marine biological resources, and the technical research on the development of enteromorpha biomass energy is relatively backward. [0003] The ocean area is huge, and there are rich Enteromorpha resources, which have various types, high photosynthetic efficiency, and short gr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/04C12P7/10C05G3/00
CPCY02E50/10
Inventor 朱凤
Owner 邳州德好科技有限公司
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