Single-frame light intensity transmission quantitative phase microscope system and method

A phase microscopy, light intensity transmission technology, applied in microscopes, optics, measuring devices, etc., to achieve the effect of non-destructive rapid quantitative detection method, high spatial resolution, and reduced measurement time

Inactive Publication Date: 2015-02-11
NANJING UNIV OF SCI & TECH
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Problems solved by technology

[0007] The purpose of the present invention is to provide a single-frame light intensity transmission quantitative phase microscopy system and method thereof, to solve the quantitative phase microscopic imaging problem based on the light intensity transmission equation, and

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  • Single-frame light intensity transmission quantitative phase microscope system and method
  • Single-frame light intensity transmission quantitative phase microscope system and method
  • Single-frame light intensity transmission quantitative phase microscope system and method

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[0024] Combine image 3 The single-frame light intensity transmission quantitative phase microscopy system of the present invention is composed of two parts, one is a microscopic imaging system 1, and the other is a Michelson-structured spectroscopic system 2 containing a spatial light modulator. The points in the figure are marked by dashed boxes. The microscopic imaging system 1 includes a condenser 3, a condenser aperture diaphragm 4, a condenser lens 5, a sample to be tested 6, a microscope objective lens 7, a first reflecting mirror 8, and a tube lens 9, wherein the condenser lens 3 Converge the illumination light to the condenser aperture diaphragm 4. The size of the condenser aperture diaphragm 4 is adjustable to control the clear aperture of the illumination. The light diverges through the condenser aperture diaphragm 4 and is collected by the condenser 5 to illuminate the sample and transmit the light through the sample. It is collected by the microscope objective lens...

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Abstract

The invention discloses a single-frame light intensity transmission quantitative phase microscope system and method. The light splitting is realized by utilizing a Michelson structure, digital defocus can be carried out by adopting a space light modulator, the mechanical movement caused by adopting a defocus strength image in a traditional method can be avoided, the acquired series of light intensity is distributed, and the quantitative acquisition of the phase information can be realized by solving the light intensity transmission equation. By adopting the system and the method, the mechanical movement caused by acquiring the defocus strength image in the traditional method can be avoided, the acquisition speed and accuracy of the system can be greatly improved, the application range of the light intensity transmission equation method can be effectively enlarged from a static slowly-varied object to a high-speed dynamic object, and the high-speed and high-resolution three-dimensional quantitative phase microscope imaging can be realized.

Description

technical field [0001] The invention belongs to optical measurement and imaging technology, in particular to a dynamic quantitative microscopic imaging device and method based on light intensity transmission equation. Background technique [0002] Phase recovery is an important topic in optical measurement and imaging technology, and phase imaging technology is playing an important role in both biomedical and industrial detection fields. Throughout the progress of optical measurement for nearly half a century, the most classic phase measurement method should be none other than interferometry. However, the disadvantages of interferometry are also very obvious: interferometry generally requires a highly coherent light source (such as a laser), which requires a more complex interferometric device; the introduction of an additional reference optical path leads to very harsh requirements for the measurement environment; The speckle coherent noise introduced by highly coherent li...

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Application Information

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IPC IPC(8): G01B11/24G02B21/36
Inventor 陈钱左超冯世杰孙佳嵩胡岩陶天阳顾国华张玉珍喻士领张良张佳琳
Owner NANJING UNIV OF SCI & TECH
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