Technology for improving activity of coated perinuclear-factor antigen

Abstract

A provided technology for improving activity of a coated perinuclear-factor antigen comprises the following steps: (1) getting a buccal mucosa cell to be shed from a normal person, performing separation purification, and selecting a smearing method to coat the interior of a pore corresponding to slide glass with the buccal mucosa cell; and (2) adjusting the temperature of a constant-temperature drying box to be 35 + / - 5 DEG, drying the smeared cell slide glass in the drying box at the constant temperature for 40 min-100 min, so as to obtain the dried finished product which is the buccal mucosa cell slide glass, in other words, to obtain the coated perinuclear-factor antigen. By changing the processing manner of the antigen, the high-grade structure of the antigen is preserved, more antigen determinants are kept, more specific antibodies are conjugated, and thus the reaction activity of the antigen is effectively raised.

Description

technical field [0001] The invention relates to a process method for enhancing the antigenic activity of the coated perinuclear factor. Background technique [0002] At present, immunological detection technology and its products have been widely used in many detection fields such as major infectious diseases, autoimmune diseases, tumor markers, food safety and prenatal and postnatal care. In adsorption tests, enzyme-linked immunospot assays, and colloidal gold rapid detection tests, the coating of antigens is an essential and important link before detection. At present, the coating of antigens is based on the characteristics of the antigen itself, such as the isoelectric point, its own structure, the charge, etc., and the corresponding coating method is selected. The result of the coating is that the antigen is adsorbed on the surface of the coating carrier through physical or chemical action. , allowing subsequent immune responses to proceed. The problem ignored by the e...

AI Technical Summary

Problems solved by technology

The problem ignored by the existing coating technology is the change of the spatial structure after the antigen is adsorbed on the surface of the carrier. This change will cause a part of the antigenic determinant on the surface of the antigen to be buried inside the antigen structure and cannot interact with the corresponding specific antibody. Binding, the resulting number of specific binding antibodies will decrease, eventually leading to a decrease in the level of detection sensitivity

Images