Method for Separation of Biomacromolecules Using In-column Flow Stationary Phase Using Electromagnetic Field
A biomacromolecule and separation method technology, applied in the field of separation of micro-scale substances, can solve the problems of low separation degree, complex device structure and manufacturing process, difficult online detection of samples, etc., and achieve simple separation method and high separation accuracy Effect
Active Publication Date: 2015-12-30
YANBIAN UNIV
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Problems solved by technology
However, the device structure and manufacturing process of this type of technology are relatively complicated, and the equipment manufacturing precision is required to be high. For the separation of multiple components, the device preparation is more difficult.
The multi-outlet design feature is difficult to realize the online detection of samples, so it is not suitable for the separation and analysis of unknown or complex samples
In addition, most micro-separation techniques have lower resolution than existing chromatographic techniques
Therefore, the practical application of continuous separation technology must face some challenges
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[0014] Figure 1 to image 3 As shown, the method for separating biomacromolecules using an electromagnetic field in-column flow stationary phase provided by the present invention includes the following steps: selecting and synthesizing magnetic nanoparticles 1 whose surface has been modified, and the particle diameter of the magnetic nanoparticles 1 is 50nm-100nm; Turn on the pump to feed the mobile phase, turn on the power supply 3 to energize the coil 4 to form an electromagnetic field inside the separation column 5; inject the modified magnetic nanoparticles 1 into the separation column 5, and the magnetic nanoparticles 1 will stay in the separation column due to the effect of the magnetic field Inside the separation column 5, under the action of the thrust of the mobile phase and the magnetic field force of the electromagnetic field, a dynamic balance of forces is achieved, thereby reciprocating flow inside the separation column 5; biological samples with different properti...
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Abstract
The invention discloses a method for separating bio-macromolecules of a flow type stationary phase in a column by utilizing an electromagnetic field. According to mechanism of the method, surface modified magnetic nano-particles are introduced into a separation column, and an external electromagnetic field is applied, the direction of a magnetic field force of the magnetic nano-particles needs to be opposite to the thrust of a mobile phase, the magnetic nano-particles can reach a dynamic balance of force inside the column and flow back and forth inside the column when the magnetic force of the magnetic nano-particles is equivalent to the thrust of the mobile phase, target bio-macromolecules to be separated and functional groups on the surface of the particles perform adsorption and desorption effects after being added inside the column, different bio-macromolecules respectively flow out from the separation column according to different from acting force of the magnetic nano-particles, so that the aim of separation is achieved. The method has the advantage of simply and accurately separating different biological samples according to properties of the biological samples.
Description
technical field [0001] The invention relates to a method for separating microscale substances and special equipment thereof, in particular to a method for separating biological macromolecules using an electromagnetic field-in-column flow stationary phase that integrates magnetic field separation technology and chromatographic technology. Background technique [0002] At present, the separation and screening of micro-scale substances such as proteins, cells and other biological macromolecules has become a preferred main research content in various research fields. Traditional separation techniques are difficult to separate samples with a wide range of sizes at the same time, and it is difficult to obtain target substances of a single size, which will bring inconvenience to follow-up research. In order to realize the separation of biomacromolecules, some non-blocking and non-filling separation systems have been established, and most of them are continuous separation technologi...
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IPC IPC(8): G01N30/10
Inventor 李东浩朴吉寿王思宏
Owner YANBIAN UNIV