Label-free rapid optical detection method of three-dimensional distribution of age spots
A three-dimensional distribution and optical detection technology, applied in the field of optical detection, can solve the problems of strong dependence on human factors, long data acquisition time, and inability to obtain complete three-dimensional information of senile plaque distribution, etc., to achieve the effect of shortening the time.
Active Publication Date: 2015-02-25
HUAZHONG UNIV OF SCI & TECH
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Problems solved by technology
[0004] The purpose of the present invention is to overcome the disadvantages of existing senile plaque detection methods that require the use of exogenous dyes, which result in strong dependence on human factors, long data acquisition time, and inability to obtain complete three-dimensional information on the distribution of senile plaques, and provide a method for detecting senile plaques in tissues or organs. Label-free rapid optical detection method based on three-dimensional distribution information of senile plaques
Method used
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Experimental program
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Effect test
Embodiment
[0022] 1. An App / PS1 double transgenic Alzheimer's disease pathological model mouse was anesthetized and then directly immersed in liquid nitrogen to make the brain of the mouse stiff.
[0023] 2. After separating the head of the mouse from the body, first add the embedding agent (absolute ethanol: glycerol: double distilled water = 1:3:6) to liquid nitrogen to rapidly cool down to form an ice-like solid and then heat up to form a paste , it will become an emulsion after continuing to heat up, which is the best form for embedding at this time. Put the previously fixed mouse brain into the embedding agent, and immediately add liquid nitrogen to harden the embedding agent quickly, so that the embedding agent and the mouse brain become a whole.
[0024] 3. Place the fixed mouse head on a milling machine to process a flat section, and the milling machine rotates at a speed of 400r / min.
[0025] 4. Use a mercury lamp as a light source, after being filtered by a filter with a cente...
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The invention relates to a label-free rapid optical detection method of three-dimensional distribution of age spots. The label-free rapid optical detection method is characterized by comprising the following steps: (1) firstly freezing to-be-detected tissues or organs in a low-temperature environment to ensure that the tissues or organs are hardened; (2) fixing the frozen tissues or organs into a low-temperature environment in which the tissues can be mechanically processed, and performing mechanical processing to obtain tissue slices or flat sections; (3) detecting an auto-fluorescence signal of the tissue slices or the flat sections by using a photoelectric detection method to obtain information about the position distribution of the age spots; and (4) repeating the steps (2) and (3) until data of the whole tissues or organs are completely acquired, thereby obtaining information about the three-dimensional distribution of the age spots. Compared with a conventional method, the method disclosed by the invention can be used for significantly shortening the time in a whole process from preparation of samples to acquisition of age spot distribution information through imaging; and complete three-dimensional information of age spot distribution can be acquired by using the method.
Description
technical field [0001] The invention relates to the field of optical detection, in particular to a label-free rapid optical detection method for senile plaque distribution based on low-temperature optical imaging technology. Background technique [0002] The mass production of senile plaques is one of the common pathological features of neurodegenerative diseases such as Alzheimer's disease and Down syndrome. The formation of senile plaques is an important criterion for Alzheimer's disease. The distribution range and density of senile plaques in the brain change with the development of the disease. Therefore, the detection of senile plaques is of great significance for revealing the pathogenesis of these degenerative diseases and promoting the diagnosis and treatment of diseases. [0003] Existing detection methods for senile plaques are mainly based on methods such as silver staining, immunohistochemical staining, and immunofluorescence staining of isolated brain slices. ...
Claims
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IPC IPC(8): G01N21/64
Inventor 骆清铭袁菁王安乐
Owner HUAZHONG UNIV OF SCI & TECH