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Serum ammonia detection reagent

A technology for detecting reagents, serum ammonia, applied in the field of medical testing, to achieve the effects of preventing inhibition, increasing stability, and high accuracy

Active Publication Date: 2015-02-25
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The conventional ammonia detection reagent (glutamate dehydrogenase two-point method) is three reagents, which mainly have the following defects: 1. It is not suitable for common automatic biochemical analyzers. At present, the automatic biochemical analyzers used in medical inspection institutions are only Two reagent disks, so the three-reagent detection kit cannot be directly operated fully automatically; 2. The use process is cumbersome
Before use, the liquid a and liquid b in reagent 1 must be manually mixed according to a certain ratio to perform fully automatic operation, which greatly increases the workload of the operator; 3. It is easy to cause operational errors during manual operation, and there are inevitable operating error

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A serum ammonia detection reagent comprises reagent 1 and reagent 2 with a volume ratio of 6:1;

[0052] The components of reagent 1 are:

[0053] tris buffer (pH=8.0) 10mmol

[0054] Lactate dehydrogenase 2KU

[0055] γ-Fe 2 o 3 Nanoparticles 50mmol

[0056] EDTA 5.3 mmol

[0057] Reduced Coenzyme I 0.2 g

[0058] Bovine serum albumin 0.5 g

[0059] Adenosine diphosphate 0.6 mmol

[0060] Add deionized water to 1L;

[0061] The components of reagent 2 are:

[0062] tris buffer (pH=8.0) 50mmol

[0063] α-ketoglutarate 30 mmol

[0064] Glutamate Dehydrogenase 25 KU

[0065] Bovine serum albumin 0.5 g

[0066] Adenosine diphosphate 0.6 mmol

[0067] Make up to 1 L with deionized water.

[0068] Among them, γ-Fe 2 o 3 The particle size of the nanoparticles is 3 nm.

Embodiment 2

[0070] A serum ammonia detection reagent comprises reagent 1 and reagent 2 with a volume ratio of 6:1;

[0071] The components of reagent 1 are:

[0072] Tris buffer (pH=8.0) 130mmol

[0073] Lactate dehydrogenase 2KU

[0074] γ-Fe 2 o 3 Nanoparticles 25mmol

[0075] EDTA 5.3 mmol

[0076] Reduced Coenzyme I 0.2 g

[0077] Bovine serum albumin 0.8 g

[0078] Adenosine diphosphate 0.4 mmol

[0079] Add deionized water to 1L;

[0080] The components of reagent 2 are:

[0081] Tris buffer (pH=8.0) 25mmol

[0082] α-ketoglutarate 30 mmol

[0083] Glutamate Dehydrogenase 25 KU

[0084] Bovine serum albumin 0.7g

[0085] Adenosine diphosphate 0.4 mmol

[0086] Make up to 1 L with deionized water.

[0087] Among them, γ-Fe 2 o 3 The particle size of the nanoparticles is 28 nm.

Embodiment 3

[0089] A serum ammonia detection reagent comprises reagent 1 and reagent 2 with a volume ratio of 6:1;

[0090] The components of reagent 1 are:

[0091] tris buffer (pH=8.0) 50mmol

[0092] Lactate dehydrogenase 2KU

[0093] γ-Fe 2 o 3 Nanoparticles 10mmol

[0094] EDTA 5.3 mmol

[0095] Reduced Coenzyme I 0.2 g

[0096] Bovine serum albumin 1 g

[0097] Adenosine diphosphate 0.1 mmol

[0098] Add deionized water to 1L;

[0099] The components of reagent 2 are:

[0100] tris buffer (pH=8.0) 10mmol

[0101] α-ketoglutarate 30 mmol

[0102] Glutamate Dehydrogenase 25 KU

[0103] Bovine serum albumin 1g

[0104] Adenosine diphosphate 0.1 mmol

[0105] Make up to 1 L with deionized water.

[0106] Among them, γ-Fe 2 o 3 The particle size of the nanoparticles is 50 nm.

[0107] A method of using a serum ammonia detection reagent of the present invention is: select a fully automatic biochemi...

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Abstract

The invention discloses a serum ammonia detection reagent which comprises a reagent 1 and a reagent 2 according to a volume ratio being 6 to 1, wherein the reagent 1 comprises a tris buffer solution with the pH value being 8.0, lactic dehydrogenase, nano particles, ethylenediamine tetraacetic acid, reduced coenzyme I, bovine serum albumin and adenosine diphosphate; and the reagent 2 comprises a tris buffer solution with the pH value being 8.0, 2-ketoglutaric acid, glutamate dehydrogenase, bovine serum albumin and adenosine diphosphate. The reagent disclosed by the invention refers to dual reagents, has the excellent performances of being simple and convenient to operate, high in stability and high in accuracy and being generally suitable for various models of fully automatic biochemical analyzers and is a novel ammonia detection reagent which is worthy of popularization.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a reagent for detecting serum ammonia. Background technique [0002] The content of plasma ammonia in the human body is very small, but ammonia is toxic to the human body and can affect the metabolism of nerve cells. An increase in the source of blood ammonia and a decrease in outlets will both cause blood ammonia to increase. Normally, ammonia is converted to urea in the liver. In severe liver disease, ammonia cannot be cleared from the circulation, causing increased blood ammonia, high blood ammonia is neurotoxic, and can cause hepatic encephalopathy (hepatic coma), so adult blood ammonia determination is extremely important for the diagnosis and differentiation of hepatic encephalopathy significance. In addition, blood ammonia measurement is very useful in the pediatric diagnosis of Reye's syndrome, which is characterized by severe hypoglycemia, massive hepatic n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52G01N21/33
CPCG01N21/33G01N33/52
Inventor 靖百谦张永英谭柏清甘宜梧王绮赵新肖慧
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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