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Application of basil polysaccharide as antineoplastic chemotherapeutic drug synergist in preparing antineoplastic drugs

A technology of anti-tumor drugs and basil polysaccharides, which is applied in the direction of anti-tumor drugs, drug combinations, medical preparations containing active ingredients, etc., can solve the problem of enhancement, and achieve the effect of inhibiting tumor proliferation and activity

Active Publication Date: 2015-03-04
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The role of basil polysaccharides in anti-tumor metastasis has been reported in the literature and can be used for a variety of tumor metastasis. However, no reports have shown that basil polysaccharides can enhance the efficacy of various chemotherapeutic drugs in treating tumors.

Method used

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  • Application of basil polysaccharide as antineoplastic chemotherapeutic drug synergist in preparing antineoplastic drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Effects of Basil Polysaccharide on Three Kinds of Tumor Cells

[0017] Experimental materials: basil polysaccharide is the finished product of basil polysaccharide obtained by the extraction and separation method provided by the present invention; human lung cancer cell line A549 and human laryngeal cancer cell line Hep -2 and human gastric cancer cell line SGC-7901.

[0018] Experimental method: Dissolve the finished basil polysaccharide in an appropriate amount of deionized water to prepare a solution with a concentration of 10 mg / ml, sterilize it, subpackage it and store it in a -20°C refrigerator for later use. Human lung cancer cell line A549, human laryngeal cancer cell line Hep-2, and human gastric cancer cell line SGC-7901 were routinely cultured in RPMI1640 medium containing 10% newborn bovine serum (NBS), at 37°C, 5% CO 2 , cultured in a cell culture incubator with saturated humidity. Digest and passage with 0.25% trypsin. Take the cultured logarithmic grow...

Embodiment 2

[0023] In Vitro Pharmacodynamic Study on the Synergistic Effect of Basil Polysaccharide on Cisplatin

[0024] Experimental materials: basil polysaccharide is the finished product of basil polysaccharide obtained by the extraction and separation method provided by the present invention; cisplatin for injection (freeze-dried type) was purchased from Qilu Pharmaceutical Co., Ltd., specification: 20mg; NBS) human lung cancer cell line A549, human laryngeal cancer cell line Hep-2 and human gastric cancer cell line SGC-7901 in RPMI1640 medium.

[0025] Experimental method: Dissolve the finished basil polysaccharide in an appropriate amount of deionized water to prepare a solution with a concentration of 10 mg / ml, sterilize it, subpackage it and store it in a -20°C refrigerator for later use. Cisplatin for injection (freeze-dried type) is prepared according to the drug instructions. Human lung cancer cell line A549, human laryngeal cancer cell line Hep-2, and human gastric cancer ce...

Embodiment 3

[0031] In vitro pharmacodynamic study on the synergistic effect of basil polysaccharide on pirarubicin hydrochloride

[0032] Experimental materials: except that pirarubicin hydrochloride for injection was used instead of cisplatin for injection (freeze-dried type), other materials were the same as in Example 2, and pirarubicin hydrochloride for injection was purchased from Shenzhen Wanle Pharmaceutical Co., Ltd. 10mg.

[0033] Experimental content: except that the drug to be tested added is pirarubicin hydrochloride and basil polysaccharide or pirarubicin hydrochloride, other is the same as embodiment 2. The experimental results are shown in Table 1.3.

[0034] Table 1.3 Synergistic effect of basil polysaccharides on pirarubicin hydrochloride in vitro tumor inhibition

[0035]

[0036] Note: *Compared with pirarubicin hydrochloride 0.05μg / ml group, p<0.01; △compared with pirarubicin hydrochloride 0.05μg / ml group, p<0.05.

[0037] The above experimental results show that...

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Abstract

The invention discloses novel application of the pure traditional Chinese medicine preparation basil polysaccharide, namely application of basil polysaccharide as antineoplastic chemotherapeutic drug synergist in preparing antineoplastic drugs. The chemotherapeutic drug is cis-platinum, pirarubicin HCI or fluorouracil. As proved by experimental study, basil polysaccharide can enhance the functions of multiple chemotherapeutic drugs in inhibiting proliferation and activity of tumour cells (including lung cancer, laryngocarcinoma, stomach cancer and the like) from different tissue sources; in addition, according to a basil polysaccharide extracorporal cell experiment, the working concentration is free of toxicity, and basil polysaccharide is dose-dependent on the synergism of the chemotherapeutic drugs and has a clinical application prospect.

Description

technical field [0001] The present invention specifically relates to the new application of basil polysaccharide in the preparation of synergists of anti-tumor chemotherapeutic drugs, that is, the application of basil polysaccharides in the preparation of synergists of anti-tumor chemotherapeutic drugs, which belongs to the synergistic effect of traditional Chinese medicine on tumor chemotherapeutic drugs. technology field. Background technique [0002] Tumor is a disease that seriously endangers human health and life. Chemotherapy is a systemic treatment that uses chemical drugs to kill cancer cells, and is widely used in the clinical treatment of cancer patients. However, while killing cancer cells, it will inevitably damage the normal cells of the human body, resulting in a variety of adverse reactions, affecting the survival time and quality of life of cancer patients. Enhancing the efficacy of chemotherapy drugs, improving the quality of life of patients, and prolongi...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61K33/24A61P35/00A61K31/513A61K31/7048
CPCA61K31/513A61K31/7048A61K31/715A61K33/24A61K2300/00
Inventor 朱庆均肖丽张丹李路于华静
Owner SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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