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A hippocampus and tail-docking technology, which is applied in diagnosis, diagnostic recording/measurement, medical science, etc., can solve the problems of easy blood coagulation, tail docking stimulation, and less blood collection volume, and achieve increased blood collection volume, strong stability, and simple operation Effect
Inactive Publication Date: 2015-03-25
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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Problems solved by technology
[0005] The technical problem to be solved by the present invention is to provide a method for blood collection by docking the hippocamp
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Embodiment 1
[0026] (1) Prepare fish blood anticoagulant: take 0.48g of citric acid, 1.32g of sodium citrate, 1.47g of glucose, and 100ml of distilled water, fully dissolve and store at 4°C.
[0027] (2) Use a pipette to take 0.5ml of anticoagulant into a 1.5ml centrifuge tube.
[0028] (3) Take one tail each of the male and female gray hippocampus (Hippocampus erectus) from the breeding tank, blot the water on the body surface with gauze, measure the body height and wet weight, and cut off a section of tail peduncle with dissecting scissors sterilized by 75% medical alcohol, Cut off the length of the tail handle to account for 1 / 3 of the total length of the tail handle.
[0029] (4) Immediately after clipping the tail, insert the remaining caudal peduncle of the docked hippocampus into a centrifuge tube containing 0.5 ml of anticoagulant, and the caudal peduncle is located below the liquid level of the anticoagulant.
[0030] (5) Hold the centrifuge tube with one hand and fix the tail ha...
Embodiment 2
[0034] (1) Prepare fish blood anticoagulant: take 0.48g of citric acid, 1.32g of sodium citrate, 1.47g of glucose, and 100ml of distilled water, fully dissolve and store at 4°C.
[0035] (2) Use a pipette to take 0.5ml of anticoagulant into a 1.5ml centrifuge tube.
[0036] (3) Take 2 three-spotted hippocampus (Hippocampus trimaculatus) from the breeding tank, blot the water on the body surface with gauze, measure the body height and wet weight, cut off a section of tail peduncle with dissecting scissors sterilized with 75% medical alcohol, and cut The length of the caudal peduncle accounts for 1 / 3 of the total length of the caudal peduncle.
[0037] (4) Immediately after clipping the tail, insert the remaining caudal peduncle of the docked hippocampus into a centrifuge tube containing 0.5 ml of anticoagulant, and the caudal peduncle is located below the liquid level of the anticoagulant.
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Abstract
The invention relates to a sea horse docking blood sampling method which includes the steps that (1) a fish blood anticoagulant is prepared; (2) the fish blood anticoagulant acquired in the step (1) is placed into a centrifugal tube; (3) a live sea horse is acquired, water on the surface of the body of the sea horse is sucked and dried through gauze, and a section of the caudal peduncle is cut off through a pair of dissection scissors; (4) after the caudal peduncle is cut off, the remaining caudal peduncle is rapidly inserted below the liquid level of the centrifugal tube acquired in the step (2); (5) the caudal peduncle is held with one hand, the centrifugal tube is held with the other hand, and blood is sampled for one to two minutes; (6) after the blood is sampled, the sea horse is immersed in disinfectant and then put into seawater to be bred continuously. According to the method, an injector or a capillary tube is not needed, the blood is directly drained to the centrifugal tube containing the anticoagulant, the problem that the blood is prone to coagulation is effectively solved, and the blood sampling amount is greatly increased; the defects existing in a conventional docking blood sampling method are effectively overcome, the advantages of the conventional docking blood sampling method are well preserved, and the method similarly has the advantages that operation is simple, stability is high, and the docked sea horse can survive.
Description
technical field [0001] The invention belongs to the field of fish blood collection, in particular to a method for blood collection by tail docking of hippocampus. Background technique [0002] Blood is an extremely important tissue in animals. It has the functions of transporting nutrients, participating in the regulation of body fluids, maintaining the homeostasis of the internal environment and resisting harmful substances. Normal blood index values can reflect the normal physiological state of animals. Fish blood is closely related to the metabolism, nutrition and immune status of the body. When the fish body is stimulated by external factors and undergoes physiological changes, it will definitely be reflected in the blood indicators. Therefore, blood indicators are widely used to evaluate the health status, nutritional status and adaptation status of fish, and are important physiological indicators. [0003] Hippocampus (Hippocampus) belongs to the class of fish and ...
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