Ultrahigh-pressure extraction method for american cockroach
An extraction method and a technology for Periplaneta americana, which are applied in the field of ultra-high pressure extraction of Periplaneta americana, can solve problems such as the unseen extraction of Periplaneta americana, achieve good application prospects, treat burns and scalds, and have the effects of high yield
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Embodiment 1
[0025] The ultra-high pressure extraction method of embodiment 1 Periplaneta americana of the present invention
[0026] Weigh 1000g of Periplaneta americana, crush it, add 6 times the amount (v / w) of 60% ethanol, pack it into a flexible container and seal it. Put it in an ultra-high pressure extractor, increase the pressure to 300MPa, pressurize 5 times, each time for 5min, the temperature is 40°C, release the pressure, open the cover to take a sample, and filter. The filtrate was concentrated under reduced pressure at 60°C until there was no ethanol smell, and the relative density was 1.05 (measured at 50°C). The concentrated solution was refrigerated at 0°C for 24 hours, and the upper layer of oil was absorbed with filter paper, and the lower layer was centrifuged in a high-speed refrigerated centrifuge. The temperature is 5°C, and the rotation speed is 10000r / min. After centrifugation, the supernatant was concentrated under reduced pressure at 60° C., dried, and pulverize...
Embodiment 2
[0027] The ultra-high pressure extraction method of embodiment 2 Periplaneta americana of the present invention
[0028] Weigh 1000g of Periplaneta americana, crush it, add 7 times the amount (v / w) of 75% ethanol, pack it into a flexible container and seal it. Put it in an ultra-high pressure extractor, increase the pressure to 400MPa, pressurize 3 times, each time for 10min, the temperature is 50°C, release the pressure, open the cover to take a sample, and filter. The filtrate was concentrated under reduced pressure at 70°C until there was no ethanol smell, and the relative density was 1.08 (measured at 50°C). The concentrated solution was refrigerated at 5°C for 36 hours, and the upper layer of oil was absorbed with filter paper, and the lower layer was centrifuged in a high-speed refrigerated centrifuge. The temperature is 10°C, and the rotation speed is 10000r / min. After centrifugation, the supernatant was concentrated under reduced pressure at 70° C., dried, and pulveri...
Embodiment 3
[0029] The ultra-high pressure extraction method of embodiment 3 Periplaneta americana of the present invention
[0030] Weigh 1000g of Periplaneta americana, crush it, add 8 times the amount (v / w) of 70% ethanol, pack it into a flexible container and seal it. Put it in an ultra-high pressure extractor, increase the pressure to 400MPa, pressurize 3 times, each time for 15min, the temperature is 60°C, release the pressure, open the cover to take a sample, and filter. The filtrate was concentrated under reduced pressure at 75°C until there was no ethanol smell, and the relative density was 1.10 (measured at 50°C). The concentrated solution was refrigerated at 10°C for 48 hours, and the upper layer of oil was absorbed with filter paper, and the lower layer was centrifuged in a high-speed refrigerated centrifuge. The temperature is 15°C, and the rotation speed is 10000r / min. After centrifugation, the supernatant was concentrated under reduced pressure at 75° C., dried, and pulver...
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