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A method for extracting polysaccharide sulfate from kelp

A technology of polysaccharide sulfate and kelp, which is applied in the field of polysaccharide sulfate extraction, can solve the problems of low extraction rate, destroying the structure of polysaccharide sulfate, and difficulty in meeting production needs, so as to shorten extraction time, avoid changes, and improve product yield Effect

Inactive Publication Date: 2017-01-04
GUILIN HEYINXIANG NEW MATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional and commonly used methods for extracting polysaccharide sulfate esters mainly include acid extraction, alkali extraction and water extraction, etc. Among them, hot water extraction is the most common method. Acid and alkali extraction will destroy the structure of polysaccharide sulfate esters, and will also affect the content of sulfate radicals. ; while the extraction rate of hot water extraction is generally low, it is difficult to meet the production needs

Method used

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  • A method for extracting polysaccharide sulfate from kelp
  • A method for extracting polysaccharide sulfate from kelp

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Experimental program
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Effect test

Embodiment 1

[0019] A method for extracting polysaccharide sulfate from kelp, comprising the steps of:

[0020] 1) Raw material processing: 10 kg of dried sea-tangle is pulverized;

[0021] 2) Ultra-high pressure treatment: add 400kg of pure water to the pulverized kelp, pressure 350MPa, temperature 85°C, ultra-high pressure treatment for 4 minutes, filter after completion, and collect the filtrate;

[0022] 3) Ultrasonic treatment: The filtrate obtained in step 2) was ultrasonically treated for 10 minutes at an operating temperature of 85°C and a power of 300W. After completion, filter and collect the filtrate;

[0023] 4) Enzymolysis: After adding protease to the filtrate obtained in step 3) to decompose protein into amino acids, first filter through an ultrafiltration membrane with a molecular weight cut-off of 1000-3000, and then concentrate through a nanofiltration membrane to 1 / 5 of the original volume to obtain crude Polysaccharide concentrate, wherein, the feeding amount of protea...

Embodiment 2

[0026] A method for extracting polysaccharide sulfate from kelp, comprising the steps of:

[0027] 1) Raw material processing: crush 10kg of fresh kelp;

[0028] 2) Ultra-high pressure treatment: add 600kg of pure water to the crushed kelp, pressure 350MPa, action temperature 80°C, ultra-high pressure treatment for 2min, filter after completion, and collect the filtrate;

[0029] 3) Ultrasonic treatment: The filtrate obtained in step 2) was ultrasonically treated for 8 minutes at an operating temperature of 80°C and a power of 300W. After completion, filter and collect the filtrate;

[0030] 4) Enzymolysis: After adding protease to the filtrate obtained in step 3) to decompose protein into amino acids, first filter through an ultrafiltration membrane with a molecular weight cut-off of 1000-3000, and then concentrate through a nanofiltration membrane to 1 / 5 of the original volume to obtain crude Polysaccharide concentrated solution, wherein, the feeding amount of protease is 1...

Embodiment 3

[0033] A method for extracting polysaccharide sulfate from kelp, comprising the steps of:

[0034] 1) Raw material processing: 10 kg of dried sea-tangle is pulverized;

[0035] 2) Ultra-high pressure treatment: Add 500kg of pure water to the pulverized kelp, pressure 350MPa, temperature 70°C, ultra-high pressure treatment for 5 minutes, filter after completion, and collect the filtrate;

[0036] 3) Ultrasonic treatment: process the filtrate obtained in step 2) for 5 minutes with ultrasonic treatment at a temperature of 70° C. and a power of 300 W. After completion, filter and collect the filtrate;

[0037] 4) Enzymolysis: After adding protease to the filtrate obtained in step 3) to decompose protein into amino acids, first filter through an ultrafiltration membrane with a molecular weight cut-off of 1000-3000, and then concentrate through a nanofiltration membrane to 1 / 5 of the original volume to obtain crude Polysaccharide concentrate, wherein, the feeding amount of protease...

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Abstract

The invention discloses a method for extracting polysaccharide sulfate from laminaria japonica. The method comprises the following steps of treating raw materials, carrying out ultrahigh-pressure treatment, carrying out ultrasonic treatment, carrying out enzymolysis, purifying and drying, thereby obtaining a finished product. According to the method, two extraction manners are combined, so that the extracting time is shortened on one hand, and the product yield is increased on the other hand.

Description

technical field [0001] The invention relates to a method for extracting polysaccharide sulfate, in particular to a method for extracting polysaccharide sulfate from kelp. Background technique [0002] Kelp (Laminaria Japonica Aresch) is a large seaweed of the genus Laminaria Laminaria. Laminaria Japonica Aresch is rich in a variety of functional components and has high edible and medicinal value. Polysaccharides are widely found in animal cell membranes and In the cell walls of plants and microorganisms, there are high molecular polymers connected by aldehyde groups and ketone groups through glycoside bonds. That is, with the rapid development of analytical methods, the research on polysaccharides has aroused great interest of many scholars at home and abroad. Studies have shown that natural polysaccharides have anti-tumor and immune-enhancing effects. Therefore, the important biological effects of immune polysaccharides in natural animals and plants It has been paid more a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 周冠旻
Owner GUILIN HEYINXIANG NEW MATERIALS
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