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Detection method of straw phenolic acid compound

A detection method, straw phenolic acid technology, applied in the utilization of straw, agricultural field, to achieve the effect of reliable method, saving sample processing time, and high resolution

Inactive Publication Date: 2015-03-25
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Straw returning to the field is widely used as an effective straw disposal measure, and most of the reports affirm its good environmental and ecological effects, but its negative effects, such as the inhibition of crop germination, emergence and root production, are of great importance in agricultural and forestry ecology. It is ubiquitous in the system, and most of them have allelopathic effects

Method used

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  • Detection method of straw phenolic acid compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Accurately weigh 12 kinds of phenolic acids (gallic acid, protocatechuic acid, gentisic acid, p-hydroxybenzoic acid, chlorogenic acid, vanillic acid, caffeic acid, syringic acid, p-coumaric acid, ferulic acid, sinapic acid, Salicylic acid) standard substance 25 mg each, put all in a 25 ml volumetric flask, add methanol to dissolve, and dilute to obtain a concentration of 1 mg·ml -1 A mixed standard solution of 12 phenolic acids.

[0031] After diluting the mixed standard solution 10 times (concentration at 100μg·ml -1 ), injected into a reversed-phase high-performance liquid chromatograph, and carried out gradient elution with acetonitrile containing 0.5% acetic acid and aqueous solution containing 0.5% acetic acid as mobile phases, and the gradient elution process was 0~25min acetonitrile 5%~20% , 25~26min acetonitrile 20%~5%, 26~28min acetonitrile 5%~5%, high performance liquid chromatography is Agilent HPLC 1200, vacuum online degasser G1322A, quaternary pump G1311A...

Embodiment 2

[0036] Accurately weigh the above 12 kinds of phenolic acids (gallic acid, protocatechuic acid, gentisic acid, p-hydroxybenzoic acid, chlorogenic acid, vanillic acid, caffeic acid, syringic acid, p-coumaric acid, ferulic acid, sinapic acid , salicylic acid) standard substance 25 mg each, respectively placed in 25mL volumetric flasks, dissolved in methanol, and constant volume, to obtain a set of concentrations of 1 mg ml -1 standard solution, the standard solution is mixed with the sample solution to be tested without adding the standard solution and injected into the reversed-phase high-performance liquid chromatograph, and the chromatographic conditions in Example 1 are adopted to obtain 12 spectrograms respectively, and the 12 spectrograms are respectively Compared with the chromatogram of the sample liquid to be tested without adding the standard solution, the peak with significantly increased absorbance was identified as the peak of the phenolic acid, and the peak time was...

Embodiment 3

[0041]Accurately weigh the above 12 kinds of phenolic acids (gallic acid, protocatechuic acid, gentisic acid, p-hydroxybenzoic acid, chlorogenic acid, vanillic acid, caffeic acid, syringic acid, p-coumaric acid, ferulic acid, sinapic acid , salicylic acid) standard substance 25 mg each, put all in a 25 mL volumetric flask, add methanol to dissolve, and constant volume, the obtained concentration is 1mg·ml -1 The mixed standard solution of 12 kinds of phenolic acids; the above 1 mg·ml -1 The mixed standard solution was diluted step by step with methanol solution to obtain a concentration of 500 μg ml -1 , 250μg·ml -1 , 100μg·ml -1 , 50μg·ml -1 , 20μg·ml -1 , 10μg·ml -1 , 5μg·ml -1 , 1μg·ml -1 mixed standard solution. Inject 5 μL of mixed standard solutions of different concentrations, and detect and record the corresponding peak areas at 260nm, 270nm, 310nm and 325nm; -1 ) is the abscissa, and the peak area value Y (mAU) is the ordinate to draw a standard curve to obta...

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Abstract

The invention relates to the field of agriculture, in particular relates to the field of utilization of straw, and more in particular relates to a detection method of a straw phenolic acid compound. In accordance with main phenolic acid substances in straws from different sources, namely gallic acid, protocatechuic acid, gentisic acid, p-hydroxybenzoic acid, chlorogenic acid, vanillic acid, caffeic acid, syringic acid, p-coumaric acid, ferulic acid, sinapic acid and salicylic acid, a reverse-phase high performance liquid chromatography detection method which is capable of achieving separation and detection in one time in a relatively comprehensive manner is disclosed, and the method can be used for keeping the relative standard deviations (RSD) of retention times and peak areas of 12 phenolic acids respectively within 0.01-0.1% and 0.25-2.26%, showing good reproducibility; the minimum detection limit is 0.005-0.01ug / ml; and the method is reliable and effective.

Description

technical field [0001] The invention relates to the field of agriculture, in particular to the field of utilization of straw, and more specifically relates to a method for detecting phenolic acid compounds in straw. [0002] Background technique [0003] Straw is the general term for the stems and leaves (ears) of mature crops. Usually refers to the remaining part of wheat, rice, corn, potatoes, oilseeds, cotton, sugar cane and other crops (usually coarse grains) after harvesting the seeds. More than half of the photosynthetic products of crops exist in straw, which is rich in nitrogen, phosphorus, potassium, calcium, magnesium and organic matter, and is a multi-purpose renewable biological resource. [0004] Straw returning to the field is widely used as an effective straw disposal measure, and most of the reports affirm its good environmental and ecological effects, but its negative effects, such as the inhibition of crop germination, emergence and root production, are o...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 于建光杨四军常志州顾东祥石祖梁张传辉张斯梅顾克军许博张恒敢
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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