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Method for controlling fungal diseases in mushroom production

A technology of fungal diseases and mushrooms, applied in the field of antimicrobial compositions, can solve problems such as undiscovered resistant fungal species

Inactive Publication Date: 2015-03-25
DSM IP ASSETS BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although natamycin has been used for many years in e.g. the cheese industry, the emergence of resistant fungal species has not been observed so far

Method used

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  • Method for controlling fungal diseases in mushroom production
  • Method for controlling fungal diseases in mushroom production
  • Method for controlling fungal diseases in mushroom production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Synergistic antifungal activity of combined application of natamycin and thiophanate-methyl

[0061] To demonstrate the synergistic antifungal activity of the combination of natamycin and thiophanate-methyl against the fungus Verticillium, an in vitro assay was performed using 96-well microtiter plates. The following compositions were tested:

[0062] - control (no active ingredient),

[0063] -2.5ppm natamycin (DSM Food Specialties, Delft, Netherlands),

[0064] - 15 or 20ppm thiophanate-methyl,

[0065] -2.5ppm natamycin + 15ppm thiophanate-methyl,

[0066] -2.5ppm natamycin + 20ppm thiophanate-methyl.

[0067] After filling each well of the microtiter plate with 80 μl of PCB medium, the active ingredient was added from a separate stock solution prepared in methanol, thus giving an intermediate volume of 100 μl / well. Subsequently, 100 μl of a suspension of the fungus Verticillium verticillium prepared in PCB medium was used for inoculation, and each well was inoc...

Embodiment 2

[0074] Synergistic antifungal activity of combined application of natamycin and thiophanate-methyl

[0075] The experiment was carried out as described in Example 1, except that the following compositions were tested:

[0076] - control (no active ingredient),

[0077] -1.25ppm natamycin

[0078] - 5, 10 or 15ppm thiophanate-methyl

[0079] -1.25ppm natamycin + 5ppm thiophanate-methyl

[0080] -1.25ppm natamycin+10ppm thiophanate-methyl

[0081] -1.25ppm natamycin + 15ppm thiophanate-methyl

[0082] Furthermore, Trichoderma harzianum was used for inoculation. After incubation for 3 and 5 days at 25° C., the antifungal activity (in %) of the active ingredients alone and in combination was determined according to the method described in Example 1 .

[0083] The results in Table 2 reveal that: compared with natamycin or thiophanate-methyl alone, the active ingredient combination of natamycin+thiophanate-methyl inhibits the growth of Trichoderma harzianum more effectively. ...

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Abstract

The present invention relates to new antifungal compositions and their use in the method for controlling fungal diseases in mushrooms.

Description

technical field [0001] The present invention discloses new antimicrobial compositions for the control of fungal diseases in mushroom production. Background technique [0002] Currently, more than 20 species of mushrooms are cultivated commercially, and mushrooms are cultivated in more than 60 countries, among which China, the United States, Poland, the Netherlands and France are the most productive countries. [0003] Over the past 10 to 15 years, the mushroom industry has undergone many changes. Small, inefficient farms were closed or merged into larger, more productive farms with increased mechanization and centralized management. Within this framework, the control of outbreaks of fungal diseases is essential. Failure to control fungal disease outbreaks early on can be costly as untreated infested areas produce spores and propagules that can spread the disease to other areas of the farm, leading to severe losses in yield and productivity. [0004] The mushroom industry ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/90A01N47/34A01P3/00
CPCA01N43/90A01G18/20A01G18/50A01G18/70A01N47/34A01N2300/00
Inventor 雅克布斯·斯塔克
Owner DSM IP ASSETS BV