Caulerpenone, and preparation method and application thereof

A technology of pteridenone and compound, applied in the field of medicine, can solve problems such as severe mental and neurological symptoms, heavy mental and economic burden, and reduce the quality of life of the elderly

Active Publication Date: 2015-04-08
南通药享科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because advanced AD patients cannot take care of themselves and are often accompanied by severe mental and neurological symptoms, it not only seriously reduces the quality of life of the elderly, but also brings a heavy mental and economic burden to the family and society.

Method used

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  • Caulerpenone, and preparation method and application thereof
  • Caulerpenone, and preparation method and application thereof
  • Caulerpenone, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 The preparation of the fern alkenone

[0031] 1. Preparation of Pteris racemosus extract

[0032] (1) Preparation of extract

[0033] Frozen Pteris racemosa ( C. racemosa ) (collected from the coast of Zhanjiang, Guangdong) 5 kg (dry weight) were extracted three times by percolation with 30L 95% ethanol, each percolation for 1 day, and the extracts were combined;

[0034] (2) Preparation of extract extract

[0035] Concentrate the above extract under reduced pressure at a temperature of ≤45°C to recover ethanol to obtain 350 g of crude extract;

[0036] 2. Separation and purification

[0037] 1) Disperse the above-mentioned extract extract in water to form a suspension, and extract the suspension three times with petroleum ether (1.5L), ethyl acetate (1.5L) and n-butanol (1L) respectively, and the obtained extract reduces Concentrated under pressure to obtain petroleum ether extract extract (38g), ethyl acetate extract extract (160g) and n-butanol extrac...

Embodiment 2

[0054] Example 2 The effect of fernone on Aβ 25-35 Induced protective effect of SH-SY5Y cell injury

[0055] 1. Experimental samples and experimental methods

[0056] Preparation of the test sample solution: the test sample is the pure compound ferrenone prepared in the above-mentioned Example 1. Accurately weigh an appropriate amount of sample and prepare a solution with the desired concentration with DMSO for pharmacological activity testing.

[0057] Cell line and cell subculture: The activity test uses the SH-SY5Y cell line (purchased from ATCC (American type culture collection) in the United States). Subculture in DMEM medium containing 10% FBS in an incubator with 5% carbon dioxide at 37°C.

[0058] Cell activity test method (MTT method): the present invention uses the MTT method to test and evaluate the tested sample for Aβ 25-35 Protective effect leading to decreased viability of SH-SY5Y cells. Dehydrogenase in the mitochondria of living cells can metabolize and r...

Embodiment 3

[0066] Get the compound obtained in Example 1, add commonly used pharmaceutical excipients, and make dosage forms such as tablets, capsules, and oral preparations.

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PUM

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Abstract

The invention relates to the technical field of medicines, and relates to a steroid compound caulerpenone which is extracted and separated from C. racemesa and is novel in branch, a preparation method thereof and application to prepare nerve protective agents and medicines preventing Alzheimer's disease. In-vitro SH-SY5Y cellular-damage nerve protection effect experiment proves that the compound has obvious protective effect on SH-SY5Y cell with damaged beta-amyloid protein 25-35(Abeta-25-35) and is applicable to prepare nerve protective agents and medicines preventing Alzheimer's disease.

Description

technical field [0001] The present invention relates to the technical field of medicine, in particular to a steroidal compound with specific branched chains isolated from Pteris racemosus; 25-35 The damaged SH-SY5Y cells have protective active ingredients, so they can be used to prepare neuroprotective drugs and drugs for preventing Alzheimer's disease. Background technique [0002] Alzheimer's disease (AD) is a primary degenerative disease of the central nervous system, which affects more than 35 million people worldwide. Its clinical manifestations are progressive memory impairment, motor impairment, cognitive impairment, etc. (Prince, M. er al, World Alzheimer Report 2013 ; Alzheimer's Disease International: London, UK, 2013; pp 1?92). There are about 8 million AD patients in my country. Because advanced AD patients cannot take care of themselves and are often accompanied by severe mental and neurological symptoms, it not only seriously reduces the quality of life of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J9/00A61K31/575A61P25/28A61P25/00
CPCC07J9/00
Inventor 毛水春章海燕郭跃伟刘定权
Owner 南通药享科技有限公司
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