Aspergillus oryzae fermented liquid, corn stalk sugar liquid prepared from the fermented liquid, preparation method and use thereof

Abstract

The invention provides aspergillus oryzae fermentation liquor and a preparation method of the aspergillus oryzae fermentation liquor. The aspergillus oryzae fermentation liquor contains manganese peroxide with the enzymatic activity being 100-10000U / L, endo-cellulase with the enzymatic activity being 150-3000U / L, beta-dextranase with enzymatic activity being 150-3000U / L, and beta-mannase with enzymatic activity being 50-2000U / L. The invention further provides a process technology of corn stalk sugar liquor. The corn stalk sugar liquor uses corn stalks as main raw materials and is prepared by adding a hydrogen dioxide solution to degrade the corn stalks through the aspergillus oryzae fermentation liquor. The process technology has the advantages of being short in production cycle, small in occupied space, low in production cost and high in sugar yield. In addition, the prepared corn stalk sugar liquor can be used as a carbon source raw material for producing butanol, ethyl alcohol and other fermentation industries.

Description

technical field [0001] The invention belongs to the two fields of fermentation engineering and enzyme engineering, and in particular relates to a fermented liquid of aspergillus oryzae, a corn stalk sugar liquid prepared from the fermented liquid, a preparation method and an application. Background technique [0002] my country is a large agricultural country with an annual output of more than 600 million tons of crop straw. Except for a small amount used in textile and papermaking industries, most of them are dumped into the environment in the form of accumulation and incineration, which not only wastes resources but also causes great harm to the environment. [0003] The vast majority of industrial microorganisms are organotrophs (heterotrophs), so the most common source of energy comes from carbon sources such as carbohydrates, lipids and proteins. At the same time, the basic carbon of cells also comes from carbon sources. Most of the carbon sources used in the fermentat...

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Problems solved by technology

Moreover, the biodegradation disclosed in the prior art mainly adopts solid-state fermentation technology, which produces enzymes while degrading lignin, but the fermentation process is inevitably accompanied by cellulose degradation, which makes the sugar yield of the final product low; secondly, large Large-scale solid-state fermentation is an open fermentation method, and the fermentation cycle is very long, so the inevitable contamination of bacteria in the fermentation process, especially the pollution of toxic and harmful bacteria is difficult to control; again, the previous solid-state fermentation cycle is too long, Occupies a lot of space

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