Aspergillus oryzae fermentation liquor, corn stalk sugar liquor prepared through same, and preparation method and application of corn stalk sugar liquor

A technology of corn stalk and corn stalk powder is applied in the fields of fermentation engineering and enzyme engineering, and can solve the problems of long fermentation period, long period, and miscellaneous bacteria pollution.

Inactive Publication Date: 2015-04-22
BEIJING LYUKE TIANCHENG BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the biodegradation disclosed in the prior art mainly adopts solid-state fermentation technology, which produces enzymes while degrading lignin, but the fermentation process is inevitably accompanied by cellulose degradation, which makes the sugar yield of the final product low; secondly, large Large-scale solid-stat

Method used

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  • Aspergillus oryzae fermentation liquor, corn stalk sugar liquor prepared through same, and preparation method and application of corn stalk sugar liquor
  • Aspergillus oryzae fermentation liquor, corn stalk sugar liquor prepared through same, and preparation method and application of corn stalk sugar liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Embodiment 1 A kind of Aspergillus oryzae fermented liquid

[0076] The Aspergillus oryzae fermentation broth contains 100 U / L of manganese peroxidase activity, 150 U / L of endocellulase activity, 150 U / L of β-glucanase activity, and 50 U of β-mannanase activity. / L; the Aspergillus oryzae fermented liquid is prepared by using corn stalks as the main raw material and Aspergillus oryzae as the strain, through test tube expansion culture, liquid shake flask culture, seed tank seed culture and liquid fermentation culture, and is prepared according to a conventional method.

Embodiment 2

[0077] Embodiment 2 A kind of Aspergillus oryzae fermented liquid

[0078] The Aspergillus oryzae fermentation broth contains 1000 U / L of manganese peroxidase activity, 1000 U / L of endocellulase activity, 500 U / L of β-glucanase activity, and 200 U of β-mannanase activity. / L; Described aspergillus oryzae fermented liquid is prepared according to the following method:

[0079] a. test tube expansion culture: inoculate the Aspergillus oryzae species in the potato glucose medium, and cultivate it for 5 days at a temperature of 30° C. to obtain the Aspergillus oryzae test tube slant spores;

[0080] b. Liquid shake flask culture: Inoculate the slanted spores of Aspergillus oryzae test tubes in a shake flask equipped with a liquid shake flask medium, and cultivate them for 50 hours at a temperature of 35° C. to obtain a liquid shake flask strain;

[0081] c. Primary seed tank seed culture: inoculate the liquid shake flask strain into the seed tank culture medium, and cultivate it ...

Embodiment 3

[0083] Embodiment 3 A kind of Aspergillus oryzae fermented liquid

[0084] The Aspergillus oryzae fermentation broth contains 5000U / L of manganese peroxidase activity, 2000U / L of endocellulase activity, 1500U / L of β-glucanase activity, and 1000U of β-mannanase activity. / L; Described aspergillus oryzae fermented liquid is prepared according to the following method:

[0085] a. test tube expansion culture: inoculate the Aspergillus oryzae species in the potato glucose medium, cultivate it for 6 days at a temperature of 25° C., and then store it at 7° C. to prepare the Aspergillus oryzae test tube slant spores;

[0086] b. Liquid shake flask culture: Inoculate a ring of Aspergillus oryzae test tube slant spores into a 250mL Erlenmeyer flask containing 100mL liquid shake flask culture medium, and cultivate it on a shaker at a temperature of 32°C for 48 hours at a rotating speed of 100 rpm. , to obtain the liquid shake flask bacterial classification; the weight of each component ...

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Abstract

The invention provides aspergillus oryzae fermentation liquor and a preparation method of the aspergillus oryzae fermentation liquor. The aspergillus oryzae fermentation liquor contains manganese peroxide with the enzymatic activity being 100-10000U/L, endo-cellulase with the enzymatic activity being 150-3000U/L, beta-dextranase with enzymatic activity being 150-3000U/L, and beta-mannase with enzymatic activity being 50-2000U/L. The invention further provides a process technology of corn stalk sugar liquor. The corn stalk sugar liquor uses corn stalks as main raw materials and is prepared by adding a hydrogen dioxide solution to degrade the corn stalks through the aspergillus oryzae fermentation liquor. The process technology has the advantages of being short in production cycle, small in occupied space, low in production cost and high in sugar yield. In addition, the prepared corn stalk sugar liquor can be used as a carbon source raw material for producing butanol, ethyl alcohol and other fermentation industries.

Description

technical field [0001] The invention belongs to the two fields of fermentation engineering and enzyme engineering, and in particular relates to a fermented liquid of aspergillus oryzae, a corn stalk sugar liquid prepared from the fermented liquid, a preparation method and an application. Background technique [0002] my country is a large agricultural country with an annual output of more than 600 million tons of crop straw. Except for a small amount used in textile and papermaking industries, most of them are dumped into the environment in the form of accumulation and incineration, which not only wastes resources but also causes great harm to the environment. [0003] The vast majority of industrial microorganisms are organotrophs (heterotrophs), so the most common source of energy comes from carbon sources such as carbohydrates, lipids and proteins. At the same time, the basic carbon of cells also comes from carbon sources. Most of the carbon sources used in the fermentat...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12N9/24C12N9/08C12P19/14C12P7/16C12P7/10C12R1/69
CPCC12N9/0065C12N9/2405C12N9/2491C12P7/10C12P7/16C12P19/14C12Y111/01013C12Y302/01004Y02E50/10
Inventor 张志才
Owner BEIJING LYUKE TIANCHENG BIO TECH
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