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Compound method applied to storage and preservation of fresh date

A technology of fresh jujube and yeast is applied in the composite field of fresh jujube storage and preservation, which can solve the problems of inability to completely control postharvest diseases and the like

Inactive Publication Date: 2015-04-29
TARIM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it has been reported that acetic acid fumigation alone can reduce the postharvest rot of fruits and vegetables, it also has certain defects, so it cannot completely control postharvest diseases.

Method used

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  • Compound method applied to storage and preservation of fresh date
  • Compound method applied to storage and preservation of fresh date
  • Compound method applied to storage and preservation of fresh date

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0028] Preparation of Alternaria and Penicillium citrinum

[0029] Alternaria ( Alternaria alternata ), Penicillium citrinum ( Penicillinum citrinum ) was isolated from the laboratory, and it was the main pathogenic bacteria that caused green rot and black rot in Xinjiang fresh jujube during storage. Transfer two kinds of pathogenic bacteria to PDA respectively (weigh 200 g potatoes, wash, peel and cut into small pieces, add 1000 mL of water to boil for 0.5 h, filter with gauze, add 2% glucose and 2% agar, add water to 1000mL, sterilized at 121°C for 15 min) culture medium at 20°C for 7 days, wash the conidia in the colony with sterile water containing 0.03% Tween-20, shake them evenly and count them with a blood cell Plate to adjust the spore suspension concentration to 10 5 spores / mL.

Embodiment 1

[0030] Example 1: Determination of the Quality of Round, Crisp and Fresh Jujubes by Different Treatments

[0031] experiment method

[0032] Control group (CK): Inoculate the fruit with Penicillium citrinum or Alternaria suspension; Acetic acid fumigation treatment group (A): Inoculate the round crisp fruit with Penicillium citrinum or Alternaria suspension in (22 ±1) ℃, fumigated with 5.4mg / L acetic acid for 40 minutes, and then forced air to dry; antagonistic yeast treatment group (Magegimechisomyces) (Y): inoculated with Penicillium citrinum or Alternaria The fruit of the suspension is soaked in the yeast spore suspension (10 8 per mL) for about 2 min, and then forced ventilation immediately to cool and dry; acetic acid fumigation treatment combined with antagonistic yeast treatment group (A+Y): the fruit was first fumigated with acetic acid, and then soaked in antagonistic yeast, Once removed, allow to dry with forced air. The treated fruits of each group were placed in...

Embodiment 2

[0048] Embodiment 2: different treatment is measured to the prevention and control of round crisp fresh jujube disease

[0049] experiment method

[0050] The surface of round crisp jujube fruit was sterilized with 2% NaClO solution for about 2 min, then rinsed with water and dried for later use. Use a sterilized toothpick to pierce a 3 mm × 3 mm × 3 mm wound on the equator of the round crisp fruit surface, and then inoculate 20 μL of 10 5 sproes / mL of Penicillium citrinum or Alternaria suspension, and then dried for later use.

[0051] Control group (CK): Inoculate the fruit with Penicillium citrinum or Alternaria suspension; Acetic acid fumigation treatment group (A): Inoculate the round crisp fruit with Penicillium citrinum or Alternaria suspension in (22 ±1) ℃, fumigated with 5.4mg / L acetic acid for 40 minutes, and then forced air to dry; antagonistic yeast treatment group (Magegimechisomyces) (Y): inoculated with Penicillium citrinum or Alternaria The fruit of the sus...

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Abstract

The invention discloses a compound method applied to the storage and preservation of a fresh date. The compound method comprises the following steps: firstly screening antagonistic saccharomycete, wherein the antagonistic saccharomycete is metschnikowia pulcherrima; inoculating a bacterial colony around the saccharomycete on a culture solution of 200 mL, carrying out shaking culture at 28 DEG C for 24 hours, then collecting thalli by using a centrifugal machine, wherein the centrifugal rotational speed is 8000 r / minute, and the working time of the centrifugal machine is 10 minutes; then washing the saccharomycete thalli for twice by using sterile normal saline with the concentration of 0.85%; finally washing the saccharomycete by using the sterile normal saline to prepare a saccharomycete suspension, counting by using a blood counting chamber, regulating to required concentration by using the sterile normal saline so that the concentration of the saccharomycete suspension is 1*108 / mL, and placing into a refrigerator for preservation and later use; carrying out fumigating treatment on a round crisp fresh date fruit for 40 minutes by using 5.4 mg / L acetic acid under the condition of 22+ / -1 DEG C, airing, then immersing into the saccharomycete suspension for about 2 minutes, taking out, then ventilating and airing in a forcing way, then bagging into a freshness protection package, and placing into a refrigeration house for storage, wherein the temperature-humidity conditions of the storage are as follows: -2+ / -1 DEG C, RH 90+ / -5%.

Description

technical field [0001] The invention relates to a composite method for fresh jujube storage and preservation, in particular to a composite preservation method for round and crisp fresh jujubes through acetic acid fumigation treatment combined with antagonistic yeast. Background technique [0002] Jujube is a traditional tonic for Chinese people. It has the effects of invigorating the spleen, replenishing qi, and neutralizing the spleen. It is beneficial for people with spleen deficiency, chronic diarrhea, and frail people, as well as patients with hepatitis, anemia, and thrombocytopenia. At present, controlling the occurrence of jujube fruit diseases mainly depends on the use of chemical fungicides. For a long time, the use of chemical fungicides to control postharvest diseases of fresh jujube has been considered the most effective control method. However, with the gradual increase in the resistance of pathogenic bacteria, as well as the continuous improvement of people's ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/144A23B7/155
Inventor 郭东起杨保求陈胜惠子
Owner TARIM UNIV
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