Flow-dividing baffle for homogenate tank for filling chromatographic column

Abstract

The invention discloses a flow-dividing baffle for a homogenate tank for filling a chromatographic column, and relates to the technical field of chromatographic column filling by a high-pressure homogenate method. The flow-dividing baffle comprises a first stainless steel metal sheet, stainless steel beads, a second stainless steel metal sheet and a polymer material, wherein the stainless steel beads are arranged between the first stainless steel metal sheet and the second stainless steel metal sheet; the first stainless steel metal sheet, the stainless steel beads and the second stainless steel metal sheet are embedded into the polymer material through the injection molding technology; the flow-dividing baffle wholly adopts a cylindrical cap-shaped structure; the cylindrical cap-shaped structure can sleeve the top end of a cavity of the homogenate tank to realize a sealing effect on the homogenate tank while flow dividing. The flow-dividing baffle is simple in design, convenient to operate and easy to process, can efficiently prevent a pressurized medium from direct impact on a slurry inside the homogenate tank from an inlet, a condition that sparse homogenate generates violent turbulence in a filling process can be avoided, and the column efficiency of the chromatographic column filled through the homogenate method is greatly improved.

Description

technical field [0001] The invention relates to the technical field of high-pressure homogenization method for filling chromatographic columns, in particular to a diverter baffle for a homogenization tank used for filling chromatographic columns. Background technique [0002] At present, for high-performance liquid chromatography columns, especially the popular high-efficiency analytical columns packed with 3-10um fine-column diameter fillers, the wet method, that is, the homogenate method, must be used for column packing, and the most commonly used method is high-pressure homogenization. slurry method. Suspend the filler in a suitable homogenate to make a homogenate, and before it settles, press it into the column with a high-pressure pump at a high flow rate to prepare a uniformly packed column. During the packing process of the high-pressure homogenization method, the high-pressure pump must be able to provide a high enough packing pressure and flow rate to ensure that a...

AI Technical Summary

Problems solved by technology

During the packing process of the high-pressure homogenization method, the high-pressure pump must be able to provide a high enough packing pressure and flow rate to ensure that a complete column bed layer is formed quickly before the packing is not graded and settled during the packing process. However, if during the packing process, Instantaneous high pressure creates turbulent flow in the thin homogenate, which makes the uniform particle distribution in the homogenate disordered and cannot ensure the uniformity of the bed layer, resulting in a decrease in column efficiency.

There is no solution to this problem in the current technology, which limits the improvement of column efficiency

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