Method for purifying polypeptide PDGF receptor inhibitor

A receptor inhibitor and polypeptide technology, which is applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve the problems of large damage to salt component fillers, increased pressure, and less application of chemically synthesized peptide drugs. Simple and easy to implement, convenient for large-scale production effect

Inactive Publication Date: 2015-04-29
WUXI MTLH BIOTECH
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Reversed-phase HPLC has a high resolution, but there are problems such as repeated use, which easily increases the pressure, and the salt component damages the filler. Therefore, it is necessary to introduce a new method to pretreat the peptide and then use reversed-phase HPLC to refine it.
[0005] Ion exchange is a commonly used separation technology. With the continuous efforts of material manufacturers, more and more media have been developed and widely used in production practice. Ion exchange resin has the advantages of low price, wide working range, and organic Solvent tolerance, alkali-resistant solution in-place cleaning and other advantages, so it has become a routine purification method in the separation and purification of biotech drugs, especially recombinant drugs, but it is less used in the purification of chemically synthesized peptide drugs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Obtaining P47 Crude Peptide by Solid Phase Synthesis

[0039] Sample treatment: Dissolve P47 crude peptide with 1% ammonia solution at a ratio of 30mg / ml to make a P47 solution with a pH of 10.5. After the sample is completely dissolved, filter it with a 0.45μm filter membrane, collect the filtrate, and store it at 2-8°C for later use.

[0040] Crude extraction of ion exchange resin: Use a sodium hydroxide solution with a pH of 11 to equilibrate the resin until the pH is detected to be constant. Inject the P47 solution into the ion exchange column until all the samples enter the chromatographic medium, rinse with a sodium hydroxide solution with a pH of 11 until equilibrium, and then rebalance with a solution with a pH of 11 and a sodium chloride concentration of 100mmol / L to remove Impurities were eluted with an acetic acid solution with a pH of 4.0 after equilibration, and the main peak was collected under ultraviolet monitoring conditions to obtain a crude extract of...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to view more

Abstract

The invention relates to the field of polypeptide purification, especially to a purification method of a polypeptide PDGF receptor inhibitor prepared by a solid-phase synthesis method. According to the method, polypeptide undergoes crude extraction through ion exchange resin, and refining is carried out by high performance liquid chromatography after conversion in the form of salt. Finally, purity reaches more than 95%, and yield reaches 25%. The polypeptide purification method can be used for large-scale preparation of the polypeptide.

Description

technical field [0001] The present invention relates to the purification of a PDGF receptor inhibitor used for the treatment of tumors, organ fibrosis and other diseases. The inhibitor is composed of 47 amino acids and adopts a solid-phase synthesis process. The present invention relates to a crude peptide obtained by solid-phase synthesis Crude extraction, refined purification method. Background technique [0002] Tumor is a major disease that endangers human health. Millions of patients are infected every year, causing a large number of patients to die. Taking liver cancer as an example, as one of the highly malignant tumors, the number of patients who die of liver cancer and its complications in China is as high as 470,000 cases, the cost of liver cancer treatment has reached 20 billion yuan, seriously occupying medical resources and funds, and has become a major problem that needs to be solved urgently for effective treatment. [0003] Existing research results have sho...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/00C07K1/20C07K1/18
CPCC07K14/49
Inventor 张明义黄岚周华
Owner WUXI MTLH BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap