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Pochonia chlamydosporia strain and screening and application thereof

A technology of Purconia bacteria and spores, which is applied to chlamydia spore Purconia strains and their screening and application fields, can solve problems such as lack of resources for efficient production of nematicides and other problems, so as to suppress the number of offspring populations. , the effect of inhibiting the reproduction of pests

Active Publication Date: 2015-04-29
YUNNAN UNIV
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Problems solved by technology

[0004] An object of the present invention is to overcome the deficiencies in the existing nematicides and the shortage of strain resources for high-efficiency production of nematicides, and to screen out the nematicide-producing Auranthus aureus from fungi with the function of poisoning and killing plant pathogenic nematodes Pochonia chlamydosporia (Pochonia chlamydosporia) YMF 1.00613, a strain of secondary metabolites of aurovertins, lays the foundation for the research and development of biological nematicides and biomimetic biopesticides

Method used

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  • Pochonia chlamydosporia strain and screening and application thereof
  • Pochonia chlamydosporia strain and screening and application thereof
  • Pochonia chlamydosporia strain and screening and application thereof

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Embodiment Construction

[0019] The Chlamydobacterium chlamydosporia (Pochonia chlamydosporia) YMF 1.00613 used in this example was deposited on May 10, 2010 in the General Microorganism Center of China Committee for the Collection of Microorganisms, and the preservation number is CGMCC-3806.

[0020] The screening steps of the nematicide fungus Chlamydosporia chlamydosporia (Pochonia chlamydosporia) YMF1.00613 strain used in this embodiment are as follows:

[0021] (1) Separation and purification of strains

[0022] Root-knot nematode-infected tobacco root samples were collected from the waterpipe field in Jianshi, Yunnan, and sealed with plastic bags. Cut ±300g root knots into 3cm or 5cm sections, put them in a Baermann funnel device filled with water, connect a rubber tube below the funnel to the nematode receiving test tube, and the nematode will sink to the bottom of the tube due to gravity. After 24 hours, clamp the rubber tube, remove the receiving tube, and centrifuge at 8000 rpm to discard t...

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Abstract

The invention relates to a pochonia chlamydosporia strain and screening and application thereof, and belongs to the technical field of nematode-killing biological resources and microbial strains. The pochonia chlamydosporia YMF 1.00613 is preserved at the China General Microbiological Culture Collection Center on May 10, 2010; and the preservation number is CGMCC No.3806. The bacterial strain is screened by the following steps: separating and purifying the bacterial strain; and screening homologous secondary metabolites of producing aurovertins of the bacterial strain. The pochonia chlamydosporia YMF 1.00613 strain disclosed by the invention is applied to fermentation production of agricultural insecticides and biopesticides for preventing and controlling plant pathogen nematode. The pochonia chlamydosporia strain has the beneficial effects that breeding of insects and quantity of offspring population can be obviously inhibited; a PDB fermentation liquor of the strain and the main component aurovertins compound of the secondary metabolites have nematode-killing capacity; the nematicidal activity under an experiment condition can be up to 90% or over; and the pochonia chlamydosporia strain can be applied to fermentation production of bio-active substances such as nematicides and biopesticides.

Description

Technical field: [0001] The invention relates to a strain of chlamydocetes sporoconiae and its screening and application, and belongs to the technical field of nematicidal biological resources and microbial strains. Background technique: [0002] Phytopathogenic nematodes are a serious plant disease. According to reports, there are more than 5,000 species of phytopathogenic nematodes in more than 200 genera. Plant pathogenic nematode diseases cause economic losses of nearly 157 billion US dollars per year to agriculture worldwide, second only to fungal diseases. At present, chemical control is still one of the important measures to control plant nematodes, but the long-term use of highly toxic chemical nematicides is increasingly harmful to the environment and human health. Therefore, the research and development of bio-nematicides with high efficiency and low toxicity has become an important issue for the sustainable development of agriculture. [0003] The biological con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/645
CPCA01N63/30C12N1/14C12N1/145C12R2001/645
Inventor 张克勤王彦利牛雪梅
Owner YUNNAN UNIV
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