Reagent strip for detecting content of creatinine in urine and preparation method of reagent strip
A reagent strip and creatinine technology are applied to the reagent strip for detecting creatinine content in urine and the field of preparation thereof, achieving the effects of sensitive color reaction, reducing false positive rate and prolonging shelf life
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Embodiment 1
[0027] A reagent strip for detecting creatinine content in urine, including a substrate and a filter paper sheet, prepared through the following steps:
[0028] Step 1) Prepare liquid A: Add 200g of β-glycerophosphate disodium, 2.5g of sodium citrate, 1.2g of copper sulfate and 0.1g of polyvinylpyrrolidone to each 1000mL of liquid A, and dilute to volume with deionized water , when the volume is constant, the pH of A solution can be controlled by adding hydrochloric acid to 6~7;
[0029] Step 2) Prepare liquid B: add 1.2g of 3,3',5,5'-tetramethylbenzidine, 80g of absolute ethanol, 2.6g of 8-hydroxyquinoline, 2g The polyvinylpyrrolidone of cumene hydroperoxide and 4g, constant volume with chloroform;
[0030] Step 3) Immerse the filter paper piece in the liquid A, take it out after fully soaking, and place it in a vacuum drying oven at a temperature of 60°C to dry for 5 minutes;
[0031] Step 4) Immerse the filter paper piece in the B solution, take it out after fully soaking...
Embodiment 2
[0034] A reagent strip for detecting creatinine content in urine, including a substrate and a filter paper sheet, prepared through the following steps:
[0035] Step 1) Prepare liquid A: Add 250g of β-glycerophosphate disodium, 3.5g of sodium citrate, 2.0g of copper sulfate and 0.5g of polyethylene glycol to each 1000mL of liquid A, and set the concentration with deionized water. When the volume is constant, the pH of A solution can be controlled by adding hydrochloric acid to 6~7;
[0036] Step 2) Prepare liquid B: add 1.8g of 3,3',5,5'-tetramethylbenzidine, 120g of absolute ethanol, 3.2g of 2-hydroxyquinoline, 3g Dicumyl hydroperoxide and the polyethylene glycol of 8g, constant volume with chloroform;
[0037] Step 3) Immerse the filter paper piece in the liquid A, take it out after fully soaking, and place it in a vacuum drying oven at a temperature of 80°C to dry for 20 minutes;
[0038] Step 4) Immerse the filter paper piece in the B solution, take it out after fully so...
Embodiment 3
[0041] A reagent strip for detecting creatinine content in urine, including a substrate and a filter paper sheet, prepared through the following steps:
[0042] Step 1) Prepare liquid A: Add 250g of β-glycerophosphate disodium, 3.5g of sodium citrate, 2.0g of copper sulfate and 0.5g of polyethylene glycol to each 1000mL of liquid A, and set the concentration with deionized water. When the volume is constant, the pH of A solution can be controlled by adding hydrochloric acid to 6~7;
[0043] Step 2) Prepare liquid B: add 1.8g of 3,3',5,5'-tetramethylbenzidine, 120g of absolute ethanol, 3.2g of 8-methylquinoline, 2.5g of tert-butyl hydroperoxide and 8g of polyvinylpyrrolidone, constant to volume with chloroform;
[0044] Step 3) Immerse the filter paper piece in the liquid A, take it out after fully soaking, and place it in a vacuum drying oven at a temperature of 80°C to dry for 20 minutes;
[0045] Step 4) Immerse the filter paper piece in the B solution, take it out after f...
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