Reagent strip for detecting content of creatinine in urine and preparation method of reagent strip

A reagent strip and creatinine technology are applied to the reagent strip for detecting creatinine content in urine and the field of preparation thereof, achieving the effects of sensitive color reaction, reducing false positive rate and prolonging shelf life

Inactive Publication Date: 2015-04-29
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention proposes an improvement to the technical solution of Chinese p

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A reagent strip for detecting creatinine content in urine, including a substrate and a filter paper sheet, prepared through the following steps:

[0028] Step 1) Prepare liquid A: Add 200g of β-glycerophosphate disodium, 2.5g of sodium citrate, 1.2g of copper sulfate and 0.1g of polyvinylpyrrolidone to each 1000mL of liquid A, and dilute to volume with deionized water , when the volume is constant, the pH of A solution can be controlled by adding hydrochloric acid to 6~7;

[0029] Step 2) Prepare liquid B: add 1.2g of 3,3',5,5'-tetramethylbenzidine, 80g of absolute ethanol, 2.6g of 8-hydroxyquinoline, 2g The polyvinylpyrrolidone of cumene hydroperoxide and 4g, constant volume with chloroform;

[0030] Step 3) Immerse the filter paper piece in the liquid A, take it out after fully soaking, and place it in a vacuum drying oven at a temperature of 60°C to dry for 5 minutes;

[0031] Step 4) Immerse the filter paper piece in the B solution, take it out after fully soaking...

Embodiment 2

[0034] A reagent strip for detecting creatinine content in urine, including a substrate and a filter paper sheet, prepared through the following steps:

[0035] Step 1) Prepare liquid A: Add 250g of β-glycerophosphate disodium, 3.5g of sodium citrate, 2.0g of copper sulfate and 0.5g of polyethylene glycol to each 1000mL of liquid A, and set the concentration with deionized water. When the volume is constant, the pH of A solution can be controlled by adding hydrochloric acid to 6~7;

[0036] Step 2) Prepare liquid B: add 1.8g of 3,3',5,5'-tetramethylbenzidine, 120g of absolute ethanol, 3.2g of 2-hydroxyquinoline, 3g Dicumyl hydroperoxide and the polyethylene glycol of 8g, constant volume with chloroform;

[0037] Step 3) Immerse the filter paper piece in the liquid A, take it out after fully soaking, and place it in a vacuum drying oven at a temperature of 80°C to dry for 20 minutes;

[0038] Step 4) Immerse the filter paper piece in the B solution, take it out after fully so...

Embodiment 3

[0041] A reagent strip for detecting creatinine content in urine, including a substrate and a filter paper sheet, prepared through the following steps:

[0042] Step 1) Prepare liquid A: Add 250g of β-glycerophosphate disodium, 3.5g of sodium citrate, 2.0g of copper sulfate and 0.5g of polyethylene glycol to each 1000mL of liquid A, and set the concentration with deionized water. When the volume is constant, the pH of A solution can be controlled by adding hydrochloric acid to 6~7;

[0043] Step 2) Prepare liquid B: add 1.8g of 3,3',5,5'-tetramethylbenzidine, 120g of absolute ethanol, 3.2g of 8-methylquinoline, 2.5g of tert-butyl hydroperoxide and 8g of polyvinylpyrrolidone, constant to volume with chloroform;

[0044] Step 3) Immerse the filter paper piece in the liquid A, take it out after fully soaking, and place it in a vacuum drying oven at a temperature of 80°C to dry for 20 minutes;

[0045] Step 4) Immerse the filter paper piece in the B solution, take it out after f...

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PUM

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Abstract

The invention discloses a reagent strip for detecting the content of creatinine in urine and a preparation method of the reagent strip. The reagent strip comprises a base plate and a filter paper piece, wherein the filter paper piece is immersed with a solution A and a solution B in sequence; the solution A contains beta-glycerophosphate, sodium citrate, copper sulfate and a surfactant, and the volume is kept constant by deionized water; and the solution B contains reductive chromogen, absolute ethyl alcohol, an antioxidant, peroxide and a surfactant, and the volume is kept constant by chloroform. According to the reagent strip, the antioxidant is added to the system so that the false positive rate of the reagent strip is reduced; meanwhile, the reagent strip can be stored for a long period of time without losing efficiency; beta-glycerophosphate is used for replacing a Tris buffering solution system and the pH value of the system is controlled to be 6-7; the peroxide is introduced so that the reagent strip is very sensitive to a developing reaction of creatinine; the developing is rapid and clear; and the gradual change difference of the colors of the reagent strips in creatinine with different concentrations is obvious, and visual judgment and machine-reading judgment of operators are facilitated.

Description

technical field [0001] The invention relates to the field of urine detection, in particular to a reagent strip for detecting creatinine content in urine and a preparation method thereof. Background technique [0002] Creatinine is the product of muscle metabolism in the human body, and it is urine creatinine when it is excreted with urine. Urinary creatinine mainly comes from the blood and is excreted from the body after being filtered by the glomerulus. The normal range of urine creatinine is 8.4~13.25mmol / 24h or 40~130mg / dl. The daily excretion of urine creatinine in normal people is very stable, basically not affected by food protein content and urine volume, when the urine creatinine value is significantly abnormal (increase or decrease), it reflects that the renal function has been damaged to a considerable extent. Therefore, clinically, by detecting changes in urine creatinine content, it is possible to control changes in diseases in a timely manner and play an impor...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 白鹏利尹焕才
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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