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Traditional Chinese medicine extract for resisting neuroblastoma as well as preparation process and application of traditional Chinese medicine extract

A technique for neuroblastoma and its preparation process, which is applied in the field of anti-neuroblastoma traditional Chinese medicine extracts to achieve the effect of inhibiting the proliferation of human bone marrow neuroblastoma cells, low production cost, good social and economic significance

Inactive Publication Date: 2015-05-06
李健
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no literature report that Osmanthus osmanthus or its extracts have anti-neuroblastoma efficacy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The preparation of embodiment 1 sweet-scented osmanthus extract

[0014] Put 1.5kg of sweet-scented osmanthus seed cake obtained after pressing to remove oil, put it in a multi-functional extraction device with a round bottom flask after crushing, add 12 times the amount of water, keep boiling after boiling, boil 3 times in total, the first time Keep boiling for 2 hours, keep boiling for 1.5 hours for the 2nd and 3rd times, combine the 3 extracts, filter, concentrate the filtrate into a thick paste, and dry at 72°C to obtain a dry product; Add 10 times the volume to mass ratio of ethyl acetate to the device, heat and reflux for extraction twice, each time for 2 hours, combine the extracts, filter, then recover the solvent under reduced pressure, dry it below 72°C to form a dry extract, and crush it into fine Powder, through a 100-mesh sieve, to obtain the Osmanthus osmanthus extract.

Embodiment 2

[0015] Example 2 Experimental study on the inhibition of the proliferation of human bone marrow neuroblastoma SH-SY5Y cells by the extract of sweet-scented osmanthus

[0016] Adherent human bone marrow neuroblastoma SH-SY5Y cells were cultured at 37°C, 5% CO 2 1. In an incubator under saturated humidity conditions, the culture medium is high-sugar methylenebisacrylamide (DMEM) with 10% fetal serum, and the cells are subcultured every 3-4 days at a ratio of 1:2. After the experiment started, the cells in the logarithmic growth phase were digested with trypsin to make a cell suspension with a concentration of 2.0×10 4 cells / ml, inoculate the cells in 96-well culture plates, add 100 μl of cell suspension to each well, so that each well contains about 2000 cells, and place the culture plates at 37°C, 5% CO 2 Incubate in an incubator for 24 h. The sweet-scented osmanthus water extract was made into a certain ratio of dilution concentration, so that the final concentration after a...

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Abstract

The invention discloses a traditional Chinese medicine extract for resisting neuroblastoma as well as a preparation process and an application of the traditional Chinese medicine extract. The traditional Chinese medicine extract, which is an extract of osmanthus fragrans lour, is obtained by extracting pressed and deoiled osmanthus fragrans lour cake by virtue of water and ethyl acetate sequentially. The traditional Chinese medicine extract disclosed by the invention, which is prepared from a single Chinese herbal medicine, is not only capable of inhibiting the proliferation of human bone marrow neuroblastoma cells, but also wide in raw material source; therefore, the traditional Chinese medicine extract is low in production cost, and is of great social and economic significance.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine extraction, and in particular relates to an anti-neuroblastoma traditional Chinese medicine extract and its preparation process and application. Background technique [0002] Neuroblastoma (neuroblastoma, NB), also known as neuroblastoma, is one of the most common malignant tumors in children that threaten children's lives. It is the fourth most common tumor after cancer. NB is a tumor of the sympathetic nervous system. It is composed of undifferentiated sympathetic nerve cells and is highly malignant. Although there have been great advances in the biological characteristics, diagnosis and treatment of NB in ​​the past 20 years, the mortality rate is still high. At present, people are actively looking for new anti-neuroblastoma drugs. On the one hand, new drugs are extracted from natural products, and on the other hand, they are explored to improve existing drugs and add new ...

Claims

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Application Information

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IPC IPC(8): A61K36/63A61P35/00A61K131/00
Inventor 李健
Owner 李健
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