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Method for extracting high-activity phellinus linteus polysaccharide at low pressure and low temperature

A technology of Phellinus linteus polysaccharide and high activity, applied in the biological field, can solve the problem of low activity of Phellinus linteus polysaccharide, and achieve the effect of high activity and increasing polysaccharide yield.

Inactive Publication Date: 2015-05-06
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the problem of low activity of Phellinus polysaccharides existing in the existing Phellinus polysaccharide extraction method, and to provide a method for extracting high-activity Phellinus polysaccharides at low temperature and low pressure that is simple to operate and can well maintain the activity of polysaccharides

Method used

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  • Method for extracting high-activity phellinus linteus polysaccharide at low pressure and low temperature
  • Method for extracting high-activity phellinus linteus polysaccharide at low pressure and low temperature

Examples

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Effect test

Embodiment 1

[0019] Add 10g Phellinus mycelium into 500mL deionized water and mix evenly, extract at a pressure of -0.04MPa and a temperature of 60°C for 2 hours, centrifuge, and use an ethanol solution with a volume fraction of 80% for the supernatant at 4°C After alcohol precipitation for 12 hours, the active Phellinus polysaccharide was obtained.

Embodiment 2

[0021] Add 10g Phellinus mycelium into 500mL deionized water and mix evenly, extract at a pressure of -0.05MPa and a temperature of 60°C for 2 hours, centrifuge and separate the supernatant with an ethanol solution with a volume fraction of 80% at 4°C After alcohol precipitation for 12 hours, the active Phellinus polysaccharide was obtained.

Embodiment 3

[0023] Add 10g Phellinus mycelia into 500mL deionized water and mix evenly, extract at a pressure of -0.06MPa and a temperature of 60°C for 2 hours, centrifuge, and use an ethanol solution with a volume fraction of 80% for the supernatant at 4°C After alcohol precipitation for 12 hours, the active Phellinus polysaccharide was obtained.

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Abstract

The invention discloses a method for extracting high-activity phellinus linteus polysaccharide at low pressure and low temperature. The method comprises the following steps: mixing male agaric mycelium obtained by a liquid fermentation method with deionized water evenly at the feed-to-liquid ratio of 1g to 40-60mL; extracting at -0.04MPa to -0.07MPa and 50-60 DEG C for 1-3 hours; and centrifuging and separating an extract liquid, and carrying out alcohol precipitation to obtain active phellinus linteus polysaccharide. The extraction method disclosed by the invention is simple, low in extraction cost, and easy to operate. Compared with a hot water extraction method and an ultrasonic assist extraction method, the yield of the phellinus linteus polysaccharide is increased; the active structure of the phellinus linteus polysaccharide can be well kept; the high-activity phellinus linteus polysaccharide is obtained.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting polysaccharide, an active ingredient of the medicinal fungus Phellinus phellidis. Background technique [0002] Phellinus igniarius, also known as Phellinus igniarius, is a fungus of the family Hymenochaetaceae and the genus Phellinus. It has anti-tumor, anti-oxidation, antibacterial, anti-inflammatory, and immune-enhancing pharmacological properties active. Phellinus is currently internationally recognized as the most effective medicinal fungus in the field of biological anti-cancer. It is the raw material for the production of anti-cancer products in the international pharmaceutical industry and health care products industry, and has a huge market potential. [0003] It is difficult for Phellinus to form Phellinus fruiting bodies in nature, and the artificial cultivation technology is immature, these are important factors affecting the yield of ...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 马小魁李乐王喆之陈康健李峻志宋双红刘陶陈勇
Owner SHAANXI NORMAL UNIV
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