Tissue culture method of stem bark of willow with buds

A technique of tissue culture and stem bark, which is applied in the field of tissue culture of willow bark with buds, can solve the problems of slow propagation speed, achieve the effects of improving survival rate, shortening propagation time, and preventing the influence of sterile environment

Active Publication Date: 2015-05-13
INST OF AGRI SCI ALONG YANGTZE RIVER IN JIANGSU
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Problems solved by technology

[0002] The willow tissue culture method is mainly a process of first producing callus, clustering buds and then rooting, and the propagation speed is relatively slow.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0020] (4) Seedling cultivation and rooting: Separate the clustered shoots and cut them into individual plants, cut the base, and insert them into the proliferation and rooting medium, the formula of the proliferation and rooting medium: 1 / 2MS+0.2mg / L 6-BA+0.8mg / L IAA+0.5mg / L NAA+1g / L activated carbon, placed in the environment of ordinary fluorescent light source, the light intensity is 1500-2000Lx, the daily light time is 20 hours, the temperature is 25±2℃, and the relative humidity is 65% -70%, cultivated for about 30 days, the rooting rate can reach 100%, and grow into 6-8cm rooted seedlings in test tubes.

[0021] (5) Seedling hardening: Store the test-tube seedlings in a bottle for 2 days, take out the test-tube seedlings and wash them, and transplant them into the substrate containing pine bark. %, it can adapt to survival after two weeks of cultivation. The survival rate is above 99%.

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Abstract

The invention discloses a tissue culture method of a stem bark of a willow with buds. The method comprises the following steps: selecting branches, and cultivating buds; selecting an explant; cultivating sterile test-tube plantlets; growing seedlings and rooting; and hardening seedlings. The tissue culture method has high practicability; the tissue culture reproduction speed and reproduction rate of the willow are improved; the reproduction speed is 2-3 times higher than those of other methods; the reproduction rate can be up to 3-5 times; and the survival rate can be ensured to be over 99%.

Description

technical field [0001] The invention relates to a tissue culture method in the field of biotechnology, in particular to a tissue culture method for willow bark with buds. Background technique [0002] The willow tissue culture method is mainly a process of producing callus first, clustering buds and then rooting, and the propagation speed is relatively slow. The method directly induces clusters from budding to rooting, shortening the propagation time. In tissue culture, when the explants are generally selected as buds, there are more chances of callus generation during the induction process. In this method, the buds with stem bark are used as the explants to improve the induction efficiency of clustered buds. Contents of the invention [0003] The purpose of the present invention is to overcome the propagation deficiency in the willow tissue culture, provide fast propagation speed, high propagation efficiency, a kind of tissue culture method of willow bark with buds with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李敏张健李玉娟
Owner INST OF AGRI SCI ALONG YANGTZE RIVER IN JIANGSU
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