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Fluorescent standard card and preparation method thereof

A standard card and fluorescent technology, applied in the field of fluorescent standard card and its preparation, can solve the problems of single excitation wavelength and unstable fluorescent substance, etc., and achieve the effect of stable fluorescent properties

Active Publication Date: 2015-05-13
SHENZHEN KINGFOCUS BIOMDICAL ENG CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide a fluorescent standard card, which solves the technical problems of unstable fluorescent substances and single excitation wavelength in the current instrument calibration process

Method used

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  • Fluorescent standard card and preparation method thereof
  • Fluorescent standard card and preparation method thereof

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Embodiment Construction

[0018] The present invention will be further elaborated below with reference to the accompanying drawings.

[0019] see figure 1 , a kind of fluorescent standard card of the present invention, it comprises a clamping shell 1, and described clamping shell 1 is strip-shaped board shape; A backing film 2, described bottoming film 2 is pasted on the surface of described clamping shell 1; A fluorescent tape 3. The fluorescent strip 3 is fixed on the substrate film 2 . The excitation light used to excite the fluorescent standard card is ultraviolet light or near ultraviolet light.

[0020] Wherein, the clamping case 1 is made of black plastic. The cartridge made of black plastic ensures that it will not be disturbed by external light when it is excited and received by ultraviolet light or near ultraviolet light, and the detection is more accurate.

[0021] Please refer to the attached figure 2 , the substrate film 2 is an NC film 4 or a PVDF film. The use of NC membrane or PVD...

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Abstract

The invention relates to a fluorescent standard card. The fluorescent standard card comprises a card housing, a bottom lining film, and a fluorescent band, wherein the card housing is strip plate-shaped; the bottom lining film is adhered to the surface of the card housing; and the fluorescent band is fixedly arranged on the bottom lining film. The fluorescent standard card provided by the invention is stable in fluorescent property. A fluorescent mixture on the standard card can satisfy calibration of an analyzer of visible band acceptance light. Moreover, the fluorescent standard card is stable for a long time, and the measurement variable coefficient CV is smaller than or equal to 0.1%.

Description

technical field [0001] The invention relates to the technical field of instrument calibration, in particular to a fluorescent standard card using fluorescent substances for calibration and a preparation method thereof. Background technique [0002] Immunofluorescence quantitative analyzer is widely used in the field of biological and biomedical testing, through the excitation, reception, and processing of specific fluorescent substances marked on the substance to be tested to analyze the content of the substance to be tested. However, such analyzers often suffer from a lack of standardization in measurements. There are several reasons for this problem: 1. The fluorescent substance used to calibrate the instrument is unstable, and fluorescence quenching, attenuation or accumulation often occurs when re-excitation. Such common substances include fluorescein, FITC, phycoerythrin, phycocyanin, quantum dots, and the like. 2. The excitation fluorescence wavelength is single, and...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 张二盈
Owner SHENZHEN KINGFOCUS BIOMDICAL ENG CO LTD
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