A kind of cultivation method of beet pollen germination
A technology of pollen germination and culture method, which is applied in the direction of plant cells, etc., can solve the problems of cumbersome agarose solid culture operation on glass slides, low germination rate of direct culture, difficult operation of pollen spotting in uncracked chemical medicine, etc., and achieves improvement In vitro culture conditions, the effect of solving the problem of low pollen germination rate, and easy control of culture conditions and methods
Inactive Publication Date: 2017-09-26
HARBIN INST OF TECH
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Problems solved by technology
[0005] The purpose of the present invention is to solve the technical problems of low germination rate of beet pollen liquid medium for direct culture, cumbersome operation of glass slide agarose solid culture, and difficult operation of pollen spotting in uncracked chemical medicine
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specific Embodiment approach 1
[0017] Specific embodiment one: a kind of beet pollen germination cultivation method, it comprises the following steps: take beet pollen, take filter paper as pollen culture carrier, place the filter paper with pollen attached in the centrifuge tube, then pour into the culture solution to infiltrate the pollen Afterwards, it is placed in a light-shielding bag or box, and cultivated at a temperature of 23° C. to 30° C. for 5 to 24 hours, thus completing the cultivation of sugar beet pollen germination.
specific Embodiment approach 2
[0018] Embodiment 2: This embodiment differs from Embodiment 1 in that: the filter paper is cut into trapezoidal paper strips with a wide top and a narrow bottom. Others are the same as in the first embodiment.
specific Embodiment approach 3
[0019] Embodiment 3: This embodiment differs from Embodiment 1 in that the area of the filter paper strip is 1 / 3 of the area of the side wall of the centrifuge tube. Others are the same as in the first embodiment.
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Abstract
The invention provides a sugar beet pollen germination cultivation method and relates to a cultivation mode and a cultivation condition through which the sugar beet pollen germination rate is increased efficiently. The method solves the technical problems that a sugar beet pollen fluid nutrient medium is low in direct germination rate, object slide agarose solid cultivation operation is tedious, and pollen sample application is not conducted easily in non-crack anther. According to the operation method, filter paper serves as a pollen adhesion carrier to be placed in an Effendorff centrifugal pipe, a centrifugal pipe cover is arranged on the centrifugal pipe after the filter paper is wetted by a microscale nutrient solution, and the filter paper is placed into a shading bag / box to be cultivated in a shading mode. By means of the method, a sugar beet pollen germination isolated cultivation mode is changed, and the sugar beet pollen isolated cultivation germination rate is increased; the method is easy to implement, the cultivation condition and mode are easy to control, and sampling cultivation can be directly conducted in the field; by means of the method, the cost is low, the cultivation time is short, and the method has wide application prospects in sugar beet pollen vitality authentication and sugar beet crossbreeding production.
Description
technical field [0001] The invention relates to the fields of sugar beet hybrid breeding and biotechnology, in particular to a cultivation method for sugar beet pollen germination. Background technique [0002] Cultivated beets include leaf beet, fodder beet, garden beet and sugar beet, and are widely used in agriculture, food and medicine. Sugar beet is a typical cross-pollination self-incompatibility crop, cross breeding is the main method of breeding offspring and breeding new varieties. Beet pollen germination culture provides pollen with strong vitality for sugar beet hybridization and artificial assisted pollination, and also provides an effective way for rapid identification of pollen germination activity and fertility in large quantities of plants. [0003] The germination rate of sugar beet pollen is not only related to the type of variety, the location of anthers, the degree of maturity of the pollen, and the time of pollen collection, but also limited by the meth...
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IPC IPC(8): C12N5/04
Inventor 刘巧红马莺程大友徐德昌罗成飞代翠红史淑芝鲁兆新张华
Owner HARBIN INST OF TECH
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